Role of microRNA-92a in metastasis of osteosarcoma cells in vivo and in vitro by inhibiting expression of TCF21 with the transmission of bone marrow derived mesenchymal stem cells
| Autor: | Lulu Shen, Shuai Cao, Liangde Jiang, Zhizheng Xiong |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Cancer Research
Proliferation medicine.disease_cause lcsh:RC254-282 Metastasis 03 medical and health sciences 0302 clinical medicine stomatognathic system Invasion In vivo microRNA MicroRNA-92a Genetics medicine lcsh:QH573-671 Migration TCF21 Osteosarcoma lcsh:Cytology Chemistry Mesenchymal stem cell Transfection lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cancer research Bone marrow Carcinogenesis Primary Research |
| Zdroj: | Cancer Cell International Cancer Cell International, Vol 19, Iss 1, Pp 1-17 (2019) |
| ISSN: | 1475-2867 |
| Popis: | Background This study aims to investigate the role of microRNA-92a (miR-92a) in metastasis of osteosarcoma (OS) cells in vivo and in vitro by regulatingTCF21 with the transmission of bone marrow derived mesenchymal stem cells (BMSCs). Methods BMSCs were isolated, purified and cultured from healthy adult bone marrow tissues. The successfully identified BMSCs were co-cultured with OS cells, and the effects of BMSCs on the growth metastasis of OS cells in vitro and in vivo were determined. qRT-PCR and western blot analysis was used to detect the expression of miR-92a and TCF21 in OS cells and OS cells co-cultured with BMSCs. Proliferation, invasion and migration of OS cells transfected with miR-92a mimics and miR-92a inhibitors was determined, and the tumorigenesis and metastasis of OS cells in nude mice were observed. Expression of miR-92a and TCF21 mRNA in tissue specimens as well as the relationship between the expression of miR-92a and the clinicopathological features of OS was analyzed. Results BMSCs promoted proliferation, invasion and migration of OS cells in vitro together with promoted the growth and metastasis of OS cells in vivo. Besides, high expression of miR-92a was found in OS cells co-cultured with BMSCs. Meanwhile, overexpression of miR-92a promoted proliferation, invasion and migration of OS cells in vitro as well as promoted growth and metastasis of OS cells in vivo. The expression of miR-92a increased significantly, and the expression of TCF21 mRNA and protein decreased significantly in OS tissues. Expression of miR-92a was related to Ennecking staging and distant metastasis in OS patients. Conclusion Collectively, this study demonstrates that the expression of miR-92a is high in OS and BMSCs transfers miR-92a to inhibit TCF21 and promotes growth and metastasis of OS in vitro and in vivo. |
| Databáze: | OpenAIRE |
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