Skin Antifreeze Protein Genes of the Winter Flounder, Pleuronectes americanus, Encode Distinct and Active Polypeptides without the Secretory Signal and Prosequences
| Autor: | Garth L. Fletcher, Zhiyuan Gong, Choy L. Hew, Zhizhou Hu, K. V. Ewart |
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| Rok vydání: | 1996 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Gene Expression Flounder Protein Sorting Signals Biology Biochemistry Antifreeze protein Antifreeze Proteins Complementary DNA Freezing Animals Amino Acid Sequence RNA Messenger Cloning Molecular neoplasms Molecular Biology Gene Chromatography High Pressure Liquid DNA Primers Gene Library Glycoproteins Skin chemistry.chemical_classification Pleuronectes Alanine Base Sequence Sequence Homology Amino Acid integumentary system cDNA library digestive oral and skin physiology DNA Cell Biology biology.organism_classification Molecular biology Recombinant Proteins digestive system diseases Amino acid Blotting Southern Liver chemistry Organ Specificity embryonic structures Winter flounder |
| Zdroj: | Journal of Biological Chemistry. 271:4106-4112 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.271.8.4106 |
| Popis: | Distinct antifreeze polypeptides (AFP) were isolated from the skin of the winter flounder, Pleuronectes americanus, by gel filtration and reverse phase high performance liquid chromatography. In parallel, several cDNA clones were isolated from a skin cDNA library using a liver AFP cDNA probe. Both protein and DNA sequence analyses indicate that flounder skin contains several distinct but homologous alanine-rich AFPs. Although the skin type AFPs contain 11 similar amino acid repeats found in the secretory liver type AFPs, the skin type AFPs are mature polypeptides lacking both the signal and prosequences, indicating that they may function intracellularly. The skin type AFP is significantly less active in thermal hysteretic activity than the liver type AFP. Genomic Southern analysis indicates that like the liver type AFP genes, there are multiple copies (30-40 copies) of skin type AFP. Although the liver type AFP genes are specifically expressed in the liver and to a lesser extent in intestine, the skin type AFP genes are expressed in all tissues examined including the liver and abundantly in exterior tissues, i.e. skin, scales, fin, and gills, suggesting an important protecting role in these exterior tissues. |
| Databáze: | OpenAIRE |
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