Simultaneous screening of the stability and dosimetry of nanoparticles dispersions for in vitro toxicological studies with static multiple light scattering technique
| Autor: | Matthias P.L. Sentis, Valérie Fessard, Gérard Meunier, Giovanni Brambilla |
|---|---|
| Přispěvatelé: | Formulaction, Toulouse, France, Laboratoire de Fougères - ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Formulaction (FORMULACTION), Formulaction |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Serial dilution Nanoparticle nanoparticule Light scattering nanomatériau 03 medical and health sciences nanotechnologie 0302 clinical medicine Suspensions test in-vitro Toxicity Tests Dosimetry Scattering Radiation toxicité Bovine serum albumin Particle Size Titanium Aqueous solution Chromatography biology nanotechnology Chemistry nanoparticle Flocculation toxicity toxicologie Serum Albumin Bovine General Medicine In vitro Culture Media in-vitro test 030104 developmental biology 030220 oncology & carcinogenesis [SDV.TOX]Life Sciences [q-bio]/Toxicology biology.protein Nanoparticles nanomaterial nanotoxicology Mass fraction nanotoxicologie toxicology |
| Zdroj: | Toxicology in Vitro Toxicology in Vitro, Elsevier, 2020, 69, pp.104972. ⟨10.1016/j.tiv.2020.104972⟩ |
| ISSN: | 0887-2333 |
| Popis: | International audience; To evaluate the nanoparticle (NP) toxicity, much efforts have been devoted for developing methods to accurately disperse NPs into aqueous suspensions prior to in vitro toxicological studies. As NP toxicity is strongly dependent on their physicochemical properties, NP characterization is a key step for any in vitro toxicological study. This study demonstrates that the static multiple light scattering (SMLS) technique allows for the simultaneous screening of the NP size, agglomeration state, stability and dosimetry in biological media. Batch dispersions of TiO2 P25 NPs in water with various bovine serum albumin (BSA) mass fractions (from 0% to 0.5%) and dilutions of these dispersions into cell culture media were characterized with SMLS. In the batch dispersions, TiO2 NPs are stable and well dispersed for BSA mass fraction lower than 0.2% while agglomeration and rapid settling is observed for higher BSA mass fractions. Paradoxically, when diluted in cell culture media, TiO2 NPs are well dispersed and stable for BSA mass fractions higher than 0.2%. The TiO2 NP dosimetry of these dilutions was evaluated experimentally with SMLS and confronted with numerical approaches. The TiO2 NP bottom concentration evolves far more slowly in the case of the higher BSA mass fraction. Such measurements give valuable insights on the NP fate and transport in biological media to obtain in fine reliable size and dose-cytotoxicity responses. |
| Databáze: | OpenAIRE |
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