A comparative study of the hypolipidaemic effects of a new polysaccharide, mannan Candida albicans serotype A, and atorvastatin in mice with poloxamer 407-induced hyperlipidaemia
| Autor: | Thomas P. Johnston, N. P. Bgatova, Alexandra B. Shintyapina, Erik Korolenko, Zuzana Nescakova, Tatyana A. Korolenko, Eva Machová, N. V. Goncharova, Marina V Khrapova, Alexander P Lykov, Ludmila V Sakhno |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Atorvastatin Pharmaceutical Science Blood lipids Down-Regulation Hyperlipidemias Poloxamer Pharmacology Polysaccharide Nitric Oxide Mannans 03 medical and health sciences Blood serum In vivo medicine Animals Candida albicans Cells Cultured Mannan Cell Proliferation chemistry.chemical_classification biology Lipid Droplets biology.organism_classification Lipids Disease Models Animal Microscopy Electron 030104 developmental biology Hexosaminidases chemistry Liver Immunology Macrophages Peritoneal Mice Inbred CBA Hydroxymethylglutaryl-CoA Reductase Inhibitors Macrophage proliferation Biomarkers medicine.drug |
| Zdroj: | The Journal of pharmacy and pharmacology. 68(12) |
| ISSN: | 2042-7158 |
| Popis: | Objectives We evaluated the hypolipidaemic effect of mannan Candida albicans serotype A, relative to atorvastatin, in a mouse model of hyperlipidaemia. Methods Mannan serotype A was investigated in vitro and in vivo to determine its effects on macrophage proliferation, nitric oxide (NO) production by cultured macrophages, serum and liver lipids, changes in liver morphology and serum chitotriosidase activity and its expression in the liver. Key findings Mannan serotype A stimulates the macrophage proliferation and NO production in murine peritoneal macrophages in vitro. The activity of serum chitotriosidase (an enzyme released from the activated macrophages) was found to be significantly increased in P-407-induced hyperlipidaemic mice pretreated with low-dose mannan compared with mice administered P-407 only. Mannan treatment in mice was shown to significantly increase the chitotriosidase expression in the liver of both non-hyperlipidaemic and P-407-induced hyperlipidaemic mice. Lastly, mice pretreated with mannan before the induction of hyperlipidaemia with P-407 showed a significant reduction in the serum concentration of atherogenic LDL cholesterol, total cholesterol, triglycerides and liver triglycerides. Conclusions It is suggested that mannan serotype A, like β-glucan, may represent another hypolipidaemic agent, which could potentially be used as an adjunctive therapy with conventional antihyperlipidaemic drugs (statins and fibrates) in humans. |
| Databáze: | OpenAIRE |
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