| Autor: |
Natalie J, Galant, Antoinette, Bugyei-Twum, Rishi, Rakhit, Patrick, Walsh, Simon, Sharpe, Pharhad Eli, Arslan, Per, Westermark, Jeffrey N, Higaki, Ronald, Torres, José, Tapia, Avijit, Chakrabartty |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Scientific Reports |
| ISSN: |
2045-2322 |
| Popis: |
Wild-type and mutant transthyretin (TTR) can misfold and deposit in the heart, peripheral nerves, and other sites causing amyloid disease. Pharmacological chaperones, Tafamidis® and diflunisal, inhibit TTR misfolding by stabilizing native tetrameric TTR; however, their minimal effective concentration is in the micromolar range. By immune-targeting sparsely populated TTR misfolding intermediates (i.e. monomers), we achieved fibril inhibition at substoichiometric concentrations. We developed an antibody (misTTR) that targets TTR residues 89–97, an epitope buried in the tetramer but exposed in the monomer. Nanomolar misTTR inhibits fibrillogenesis of misfolded TTR under micromolar concentrations. Pan-specific TTR antibodies do not possess such fibril inhibiting properties. We show that selective targeting of misfolding intermediates is an alternative to native state stabilization and requires substoichiometric concentrations. MisTTR or its derivative may have both diagnostic and therapeutic potential. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
