Highly abundant genes in the transcriptosome of human and baboon CD34 antigen-positive bone marrow cells
| Autor: | Noreen Fulton, Seby Edassery, Nadim Mahmud, Ignatius Gomes, Tiffany Sharma, Jie Liang, Carol A. Westbrook, Ronald Hoffman, Jeffrey Kapp |
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| Rok vydání: | 2001 |
| Předmět: |
DNA
Complementary Transcription Genetic Immunology CD34 Antigens CD34 Bone Marrow Cells Biology Biochemistry Species Specificity Complementary DNA Gene expression medicine Animals Humans RNA Messenger Gene Expressed Sequence Tags Genetics Expressed sequence tag Genome Gene Expression Profiling Hematopoietic stem cell Cell Biology Hematology Gene expression profiling medicine.anatomical_structure Models Animal Bone marrow Papio |
| Zdroj: | Blood. 98:93-99 |
| ISSN: | 1528-0020 0006-4971 |
| Popis: | Nonhuman primates are useful large animal model systems for the in vivo study of hematopoietic stem cell biology. To better understand the degree of similarity of the hematopoietic systems between humans and baboons, and to explore the relevance of such studies in nonhuman primates to humans, this study was designed to compare the global gene expression profile of bone marrow CD34+ cells isolated from these 2 species. Human complementary DNA (cDNA) filter arrays containing 25 920 human cDNAs were surveyed for this purpose. The expression pattern and relative gene abundance of the 2 RNA sources were similar, with a correlation coefficient of 0.87. A total of 15 970 of these cDNAs were expressed in human CD34+ cells, of which the majority (96%) varied less than 3-fold in their relative level of expression between human and baboon. Reverse transcriptase–polymerase chain reaction analysis of selected genes confirmed that expression was comparable between the 2 species. No species-restricted transcripts have been identified, further reinforcing the high degree of similarity between the 2 populations. A subset of 1554 cDNAs, which are expressed at levels 100-fold and greater than background, is described, which includes 959 expressed sequence tags and uncharacterized cDNAs, and 595 named genes, including many that are clearly involved in hematopoiesis. The cDNAs reported here represent a selection of some of the most highly abundant genes in hematopoietic cells and provide a starting point to develop a profile of the transcriptosome of CD34+cells. |
| Databáze: | OpenAIRE |
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