OpuC – an ABC Transporter that is Associated with Staphylococcus Aureus Pathogenesis
| Autor: | Andrea Giacometti, Donna E. Akiyoshi, Giorgio Scalise, Naomi Balaban, Madanahally D. Kiran, Oscar Cirioni |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Staphylococcus aureus Prosthesis-Related Infections Time Factors Virulence Factors RNAIII Colony Count Microbial 030232 urology & nephrology Biomedical Engineering Medicine (miscellaneous) Bioengineering ATP-binding cassette transporter Plasma protein binding 030204 cardiovascular system & hematology Biology Bacterial Adhesion Choline Microbiology Biomaterials 03 medical and health sciences 0302 clinical medicine Bacterial Proteins Two-Hybrid System Techniques Animals Phosphorylation Rats Wistar Adaptor Proteins Signal Transducing Regulation of gene expression Virulence Signal transducing adaptor protein Biological Transport Gene Expression Regulation Bacterial Salt Tolerance General Medicine Water-Electrolyte Balance Phosphoproteins Rats Disease Models Animal Quorum sensing Transmembrane domain Biofilms Mutation Trans-Activators ATP-Binding Cassette Transporters Carrier Proteins Protein Binding |
| Zdroj: | The International Journal of Artificial Organs. 32:600-610 |
| ISSN: | 1724-6040 0391-3988 |
| Popis: | RIP is a novel antibiotic against staphylococci. It acts at least in part by competing with RNAIII activating protein (RAP) by downregulating TRAP histidine phosphorylation, and by downregulating the expression of the Acessory Gene Regulator (agr). While much is known about the function of the agr as a quorum sensing system that regulates virulence, not much is known about TRAP. TRAP is a 167-kDa protein that is highly conserved among staphylococci and is involved in DNA protection from stress. TRAP is membrane-associated but does not have a transmembrane domain, and thus it may be bound to the membrane through other proteins. To search for these proteins, protein-protein interaction studies were carried out using a bacterial two-hybrid system, and OpuCA was discovered as a TRAP-binding protein. OpuCA is an ATP binding-cytoplasmic (ABC) domain of an OpuC ABC transporter. S. aureus OpuC– mutant strain was constructed and shown to be less tolerant to salt stress, and was defective in choline uptake. OpuC– cells were less pathogenic and showed reduced TRAP phosphorylation and agr activity, did not respond to RAP, and were defective in biofilm formation in vitro and in vivo. These results suggest that OpuC acts as a transporter and also plays a role in S. aureus pathogenesis. |
| Databáze: | OpenAIRE |
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