Si permeability of a deficient Lsi1 aquaporin in tobacco can be enhanced through a conserved residue substitution
| Autor: | Paul Isenring, Rupesh Deshmukh, Devrim Coskun, Humira Sonah, Richard R. Bélanger, Rachelle Frenette-Cotton, S. M. Shivaraj, Laurence E. Tremblay |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Arginine Xenopus Nicotiana benthamiana Aquaporin Plant Science 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) tobacco Green fluorescent protein 03 medical and health sciences influx NIP2‐1 Ecology Evolution Behavior and Systematics 030304 developmental biology Original Research 2. Zero hunger chemistry.chemical_classification 0303 health sciences Oryza sativa Ecology biology molecular determinants fungi Botany food and beverages silicon (Si) biology.organism_classification Lsi1 Amino acid aquaporin chemistry QK1-989 Biophysics Nicotiana sylvestris 010606 plant biology & botany |
| Zdroj: | Plant Direct, Vol 3, Iss 8, Pp n/a-n/a (2019) Plant Direct |
| ISSN: | 2475-4455 |
| Popis: | Silicon (Si) is a beneficial substrate for many plants, conferring heightened resilience to environmental stress. A plant's ability to absorb Si is primarily dependent on the presence of a Si‐permeable Lsi1 (NIP2‐1) aquaporin in its roots. Structure‐function analyses of Lsi1 channels from higher plants have thus far revealed two key molecular determinants of Si permeability: (a) the amino acid motif GSGR in the aromatic/arginine selectivity filter and (b) 108 amino acids between two highly conserved NPA domains. Curiously, tobacco (Nicotiana sylvestris) stands as a rare exception as it possesses an Lsi1 (NsLsi1) with these molecular signatures but is reported as a low Si accumulator. The aim of this study was therefore to identify whether additional determinants influence Si permeability via Lsi1 channels, focusing on the role of residues that differ uniquely in NsLsi1 relative to functional Lsi1 homologs. We observed tobacco indeed absorbed Si poorly (0.1% dw), despite NsLsi1 being expressed constitutively in planta. Si influx measured in NsLsi1‐expressing Xenopus oocytes was very low ( |
| Databáze: | OpenAIRE |
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