Differential expression pattern of microRNAs in CD4+ and CD19+ cells from asymptomatic patients with systemic lupus erythematosus
| Autor: | M-C Rodríguez, R Martínez-Ramos, M-J Castillo-Palma, J-M Lucena, J-R García-Lozano, Francisco J. García-Hernández, M-F González-Escribano, Antonio Núñez-Roldán |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Antigens CD19 Cell Asymptomatic CD19 Rheumatology microRNA TaqMan medicine Humans Lupus Erythematosus Systemic Differential expression Microrna array Oligonucleotide Array Sequence Analysis biology business.industry Gene Expression Profiling Middle Aged Peripheral blood body regions MicroRNAs medicine.anatomical_structure Gene Expression Regulation Case-Control Studies embryonic structures biology.protein Cancer research Female medicine.symptom business |
| Zdroj: | Lupus. 23:353-359 |
| ISSN: | 1477-0962 0961-2033 |
| DOI: | 10.1177/0961203314522335 |
| Popis: | Objective The aim of this study was to investigate the pattern of microRNA (miRNA) expression in CD19+ and CD4+ cells from asymptomatic patients with systemic lupus erythematosus (SLE). Methods A screening of the expression of 377 miRNAs was performed in human CD4+ and CD19+ cells isolated from the peripheral blood by using a TaqMan Human MicroRNA Array. Validation of differential expression pattern of those was performed using TaqMan assays in these cell populations obtained from a larger cohort of patients and controls. Results According to the screening assays, three miRNAs were differentially expressed ( p value Conclusions Our results support a different pattern of miRNA expression in SLE patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|