Neurogenic inflammation and lowering of interstitial fluid pressure in rat trachea is inhibited by alpha-trinositol
| Autor: | Rolf K. Reed, Kathrine Woie |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Rat Trachea Inositol Phosphates Drug Evaluation Preclinical Stimulation Vascular permeability Critical Care and Intensive Care Medicine Intracardiac injection Capillary Permeability Internal medicine medicine Animals Edema Rats Wistar Analysis of Variance Neurogenic inflammation Dose-Response Relationship Drug business.industry Vagus Nerve Interstitial fluid pressure Electric Stimulation Rats Trachea Alpha-trinositol Endocrinology Depression Chemical Anesthesia Circulatory system Female Tracheitis Extracellular Space business |
| Zdroj: | American Journal of Respiratory and Critical Care Medicine. 150:924-928 |
| ISSN: | 1535-4970 1073-449X |
| DOI: | 10.1164/ajrccm.150.4.7921464 |
| Popis: | The effect of alpha-trinositol (D-myoinositol-1,2,6-triphosphate) on edema formation and capillary permeability in neurogenically induced inflammatory edema was investigated in rat trachea. Interstitial fluid pressure (Pif) was studied, since increased negativity of Pif contributes to edema formation in this situation. alpha-Trinositol was used because it inhibits edema formation, capillary leakage, and increased negativity of Pif in burn-injured skin. Pif was measured with sharpened glass capillaries (3 to 7 microns) connected to a servocontrolled counterpressure system after circulatory arrest (induced by intracardiac injection of saturated potassium chloride in pentobarbital anesthesia). This was done in order to avoid the edema formation associated with inflammatory reactions, which will raise interstitial fluid volume and Pif, causing the underestimation of an increased negativity of Pif. Neurogenic inflammation induced by electrical-field stimulation of the left vagal nerve (10 V, 20 Hz, 0.5 ms) lowered Pif from -1.4 +/- 0.6 mm Hg to -8.4 +/- 2.1 mm Hg (p0.01). Corresponding numbers after the intravenous administration of alpha-trinositol (40 mg/kg) before stimulation were -1.2 +/- 0.4 and -1.4 +/- 0.4 mm Hg, respectively (p0.05). Another series of animals with intact circulation was used to study the effect of vagal nerve stimulation and alpha-trinositol on edema formation (total tissue water and extravascular 51Cr-ethylenediamine tetraacetic acid-[EDTA] space) and albumin extravasation. These parameters increased significantly after vagal nerve stimulation, while intravenous alpha-trinositol (40 and 120 mg/kg), as given above, significantly attenuated this increase. Thus, alpha-trinositol prevented a lowering of Pif and the edema formation accompanying neurogenic inflammation in rat trachea. |
| Databáze: | OpenAIRE |
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