Evaluation of vitrification protocol of mouse ovarian tissue by effect of DNA methyltransferase-1 and paternal imprinted growth factor receptor-binding protein 10 on signaling pathways
| Autor: | Xue Zhou, Zhong-yi He, Bei Yan, Rui Wang, Hong-Yan Wang, Xue-yun Liang, Liang-Hong Ma, Yi-li Wang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway DNA (Cytosine-5-)-Methyltransferase 1 medicine.medical_specialty GRB10 Adaptor Protein General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Mice Ovarian Follicle Internal medicine medicine Animals Humans RNA Messenger Phosphorylation RNA Small Interfering Protein kinase A Promoter Regions Genetic Protein kinase B Cryopreservation biology Kinase GRB10 Fertility Preservation General Medicine DNA DNA Methylation Vitrification Cell biology Mice Inbred C57BL 030104 developmental biology Endocrinology Mitogen-activated protein kinase biology.protein Oocytes Female RNA Interference Signal transduction Mitogen-Activated Protein Kinases General Agricultural and Biological Sciences Proto-Oncogene Proteins c-akt |
| Zdroj: | Cryobiology. 80 |
| ISSN: | 1090-2392 |
| Popis: | Transplantation of cryopreserved ovarian tissue has been considered as a promising way of fertility preservation for women. however, this cryopreservation method is prone to post-resuscitation follicle proliferation and oocyte development stagnation, affecting late transplant survival. To evaluate current vitrification works, we investigated the critical pathway alternations in vitrified-warmed juvenile 10-day-old mouse ovary. We showed a significant decrease of protein kinase B (Akt) and Mitogen-activated protein kinase (Mapk) phosphorylation, during which serine/threonine kinases play central roles in coordinating follicle and oocyte development and stress response. Inhibition of Akt and Mapk activity were associated with one of the imprinted insulin pathway negative regulatory genes, Growth factor receptor-binding protein 10 (Grb10) which remarkably increased in vitrified-warmed juvenile mouse ovary than that of fresh group (p 0.05). RNAi-induced Grb10 down-regulation reversed the decrease in Akt and Mapk phosphorylation. The increase of Grb10 expression was partially caused by the hyper-methylation of the promoter region, associated with the decrease of follicular DNA methyltransferase (Dnmt) 1 protein in different stages of vitrified-warmed group, compared to fresh group (p 0.05). The mRNA and protein expression of Dnmt1 in ovary of vitrified-warmed juvenile mouse were remarkably lower than those in fresh group (p 0.05). Dnmt1 overexpression dramatically reversed Grb10 up-regulation and Akt and Mapk phosphorylation reduction. Taken together, our findings suggest that Grb10 expression might be helpful in evaluation of effectiveness of vitrification, and considered as a potential target for further vitrification protocols improvement in the future. |
| Databáze: | OpenAIRE |
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