Amycolatopsis tucumanensis sp. nov., a copper-resistant actinobacterium isolated from polluted sediments
Autor: | Pablo Alonso-Vega, Virginia Helena Albarracín, Martha E. Trujillo, Maria Julia del R. Amoroso, Carlos Mauricio Abate |
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Rok vydání: | 2010 |
Předmět: |
Amycolatopsis tucumanensis
Base Sequence biology Molecular Sequence Data Amycolatopsis General Medicine biology.organism_classification Microbiology RNA Bacterial Sequence homology RNA Ribosomal 16S Sequence Homology Nucleic Acid Actinomycetales Drug Resistance Bacterial Botany Water Pollution Chemical Base sequence Water Microbiology Copper Ecology Evolution Behavior and Systematics |
Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 60:397-401 |
ISSN: | 1466-5034 1466-5026 |
Popis: | A novel actinomycete strain, ABOT, isolated from copper-polluted sediments showed remarkable copper resistance as well as high bioaccumulation abilities. Classical taxonomic methods, including chemotaxonomy and molecular techniques, were used to characterize the isolate. Strain ABOT developed a honey-yellow substrate mycelium on all ISP media tested. Abundant, white, aerial mycelium was only formed on ISP 2, 5 and 7 and MM agar. Both types of hyphae fragmented into squarish rod-shaped elements. The aerial mycelium displayed spore-like structures with smooth surfaces in long, straight to flexuous chains. The organism has a type-IV cell wall lacking mycolic acids and type-A whole-cell sugar pattern (meso-diaminopimelic acid, arabinose and galactose) in addition to a phospholipid type-II profile. 16S rRNA gene sequence studies indicated that this organism is a member of the family Pseudonocardiaceae and that it forms a monophyletic clade with Amycolatopsis eurytherma NT202T. The DNA–DNA relatedness of strain ABOT to A. eurytherma DSM 44348T was 39.5 %. It is evident from these genotypic and phenotypic data that strain ABOT represents a novel species in the genus Amycolatopsis, for which the name proposed is Amycolatopsis tucumanensis sp. nov. The type strain is ABOT (=DSM 45259T =LMG 24814T). |
Databáze: | OpenAIRE |
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