Plant-derived secretory component gives protease-resistance to Shiga toxin 1-specific dimeric IgA
| Autor: | Minami Matsuda, Shota Morikane, Yasuyuki Imai, Yasuo Niwa, Katsuhiro Nakanishi, Takeshi Matsuoka, Yuki Kikuchi, Kotome Shiina, Kohta Kurohane, Noriko Mogi, Hirokazu Kobayashi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Proteases Cell Survival Secretory component medicine.medical_treatment Plant Science Shiga Toxin 1 medicine.disease_cause 01 natural sciences 03 medical and health sciences fluids and secretions stomatognathic system Western blot Chlorocebus aethiops Genetics medicine Animals Vero Cells Protease biology medicine.diagnostic_test Toxin food and beverages Shiga toxin General Medicine Trypsin Molecular biology Immunoglobulin A Secretory Component 030104 developmental biology Immunoglobulin G Immunoglobulin A Secretory biology.protein Antibody Agronomy and Crop Science Peptide Hydrolases 010606 plant biology & botany medicine.drug |
| Zdroj: | Plant Molecular Biology. 106:297-308 |
| ISSN: | 1573-5028 0167-4412 |
| Popis: | A plant-derived secretory component conferred protease-resistance to Shiga toxin-specific mammalian immunoglobulin A. The protease-resistance was functionally demonstrated by a toxin neutralization assay. Secretory component (SC) is believed to play roles in such as the protease-resistance of secretory immunoglobulin A (SIgA), allowing it to function on mucosae. Although the use of a plant expression system for SIgA production has been increasing, it has not been sufficiently assessed as to whether heterologously expressed SC could functionally contribute to the protease-resistance of SIgA. Here, we reconstituted SIgA using plant-derived SC and tested for changes in vulnerability to protease challenge. With glutathione redox buffers, plant-derived SC and mammalian cell-derived dimeric IgA (dIgA) specific for Shiga toxin 1 (Stx1) efficiently formed SIgA. Prior ammonium sulfate precipitation of dIgA was also shown to be useful to enhance the formation of SIgA. The reconstituted SIgA was treated with two gastrointestinal proteases, pepsin and trypsin. After 2-h pepsin treatment, the signal from SIgA remained at 42% with plant-derived SC-reconstitution while that from dIgA remained at 12% without SC-reconstitution on western blot analysis. Similarly, the signal from SIgA remained at 74% with SC but that from dIgA remained at 36% without SC after 4-h trypsin treatment. Furthermore, an effect of SC-reconstitution of dIgA on pepsin-resistance was observed in a toxin neutralization assay involving Vero cells. These results indicated that the plant-derived SC could contribute to the production of orally applicable SIgA. |
| Databáze: | OpenAIRE |
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