Iodinated radiographic contrast media possess antioxidant properties in vitro
| Autor: | Per Jynge, Heidi Brurok, J. O. G. Karlsson, Morten Bruvold, Kirsti Berg, Sissel Skarra |
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| Rok vydání: | 2005 |
| Předmět: |
Xanthine Oxidase
Radiographic contrast media Antioxidant Iron Radical medicine.medical_treatment Contrast Media In Vitro Techniques chemistry.chemical_compound Triiodobenzoic Acids medicine Drug Interactions Radiology Nuclear Medicine and imaging Xanthine oxidase Radiological and Ultrasound Technology Superoxide business.industry Hydrogen Peroxide General Medicine Xanthine Iodixanol In vitro chemistry Acetylcholinesterase Colorimetry Reactive Oxygen Species Nuclear medicine business Oxidation-Reduction medicine.drug Nuclear chemistry |
| Zdroj: | Acta Radiologica. 46:815-822 |
| ISSN: | 1600-0455 0284-1851 |
| Popis: | Purpose: To study potential properties of iodinated radiographic contrast media (IRCM) for intravascular use in in vitro free radical generating reactions. Material and Methods: Superoxide (·O2−) and hydroxyl (·OH) radicals were generated in xanthine oxidase and Fenton reactions. ·O2− was assayed by the nitroblue tetrazolium (NBT) method, whereas ·OH was assayed by an aromatic hydroxylation (2-hydroxy-benzoic acid) method. Total antioxidant status (TAS) of test substances was determined by a colorimetric assay. Finally, acetyl-cholinesterase (AChE) activity was measured in the absence and presence of IRCM. Results: High concentrations (>50 mM) of IRCM inhibited ·O2− production, ionic more than non-ionic IRCM. Medium concentrations (25–50 mM) of IRCM reduced ·OH production, and both types of IRCM were equally potent. Low concentrations (® (iodixanol) was found to have the highest TAS value, followed by Omnipaque 350® (iohexol), Hexabrix 320® (ioxaglate), and Urografin 370® (diatrizoate). Conclusion: IRCM have in vitro antioxidant properties in concentrations relevant for their clinical application. These properties may therefore be of potential importance when evaluating IRCM effects in vivo, particularly those concerning cardiovascular and renal function. |
| Databáze: | OpenAIRE |
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