Human Astrocytes Model Derived from Induced Pluripotent Stem Cells
| Autor: | Shinichi Takahashi, Hirotaka Watanabe, Mitsuru Ishikawa, Kyoko Mashima, Satoru Morimoto, Hideyuki Okano, Yuta Sato, Takahiro Kondo, Kent Imaizumi, Nicolas Leventoux, Iki Sonn |
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| Rok vydání: | 2020 |
| Předmět: |
Induced Pluripotent Stem Cells
Glutamic Acid Disease Biology Models Biological Article Pathogenesis disease modeling medicine Humans Cell Lineage neurodegenerative diseases Induced pluripotent stem cell lcsh:QH301-705.5 Cells Cultured Embryoid Bodies Neurons Glutamate uptake Principal Component Analysis cell culture iPSC astrocytes RNA Cell Differentiation General Medicine Coculture Techniques In vitro medicine.anatomical_structure Microscopy Fluorescence lcsh:Biology (General) Cell culture Calcium Neuroscience Astrocyte |
| Zdroj: | Cells, Vol 9, Iss 2680, p 2680 (2020) Cells Volume 9 Issue 12 |
| ISSN: | 2073-4409 |
| Popis: | Induced pluripotent stem cell (iPSC)-based disease modeling has a great potential for uncovering the mechanisms of pathogenesis, especially in the case of neurodegenerative diseases where disease-susceptible cells can usually not be obtained from patients. So far, the iPSC-based modeling of neurodegenerative diseases has mainly focused on neurons because the protocols for generating astrocytes from iPSCs have not been fully established. The growing evidence of astrocytes&rsquo contribution to neurodegenerative diseases has underscored the lack of iPSC-derived astrocyte models. In the present study, we established a protocol to efficiently generate iPSC-derived astrocytes (iPasts), which were further characterized by RNA and protein expression profiles as well as functional assays. iPasts exhibited calcium dynamics and glutamate uptake activity comparable to human primary astrocytes. Moreover, when co-cultured with neurons, iPasts enhanced neuronal synaptic maturation. Our protocol can be used for modeling astrocyte-related disease phenotypes in vitro and further exploring the contribution of astrocytes to neurodegenerative diseases. |
| Databáze: | OpenAIRE |
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