Absence of COVID-19-associated changes in plasma coagulation proteins and pulmonary thrombosis in the ferret model

Autor: Kreft, I.C., Winiarczyk, R.R.A., Tanis, F.J., Zwaan, C. van der, Schmitz, K.S., Hoogendijk, A.J., Swart, R.L. de, Moscona, A., Porotto, M., Salvatori, D.C.F., Vries, R.D. de, Maat, M.P.M. de, Biggelaar, M. van den, Vlijmen, B.J.M. van, Dutch Covid-19 Thrombosis Coaliti
Přispěvatelé: Virology, Hematology
Rok vydání: 2022
Předmět:
Zdroj: Thrombosis Research, 210, 6. Elsevier Ltd
Thrombosis Research: Vascular Obstruction, Hemorrhage and Hemostasis, 210, 6-11. PERGAMON-ELSEVIER SCIENCE LTD
Thrombosis Research, 210, 6-11. Elsevier Ltd.
Thrombosis Research
ISSN: 0049-3848
DOI: 10.1016/j.thromres.2021.12.015
Popis: Background: Many patients who are diagnosed with coronavirus disease 2019 (COVID-19) suffer from venous thromboembolic complications despite the use of stringent anticoagulant prophylaxis. Studies on the exact mechanism(s) underlying thrombosis in COVID-19 are limited as animal models commonly used to study venous thrombosis pathophysiology (i.e. rats and mice) are naturally not susceptible to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Ferrets are susceptible to SARS-CoV-2 infection, successfully used to study virus transmission, and have been previously used to study activation of coagulation and thrombosis during influenza virus infection. Objectives: This study aimed to explore the use of (heat-inactivated) plasma and lung material from SARS-CoV-2-inoculated ferrets studying COVID-19-associated changes in coagulation and thrombosis. Material and methods: Histology and longitudinal plasma profiling using mass spectrometry-based proteomics approach was performed. Results: Lungs of ferrets inoculated intranasally with SARS-CoV-2 demonstrated alveolar septa that were mildly expanded by macrophages, and diffuse interstitial histiocytic pneumonia. However, no macroscopical or microscopical evidence of vascular thrombosis in the lungs of SARS-CoV-2-inoculated ferrets was found. Longitudinal plasma profiling revealed minor differences in plasma protein profiles in SARS-CoV-2-inoculated ferrets up to 2 weeks post-infection. The majority of plasma coagulation factors were stable and demonstrated a low coefficient of variation. Conclusions: We conclude that while ferrets are an essential and well-suited animal model to study SARS-CoV-2 transmission, their use to study SARS-CoV-2-related changes relevant to thrombotic disease is limited.
Databáze: OpenAIRE
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