A novel oleaginous yeast Saccharomyces cerevisiae CU-TPD4 for lipid and biodiesel production
| Autor: | Monnat Theerachat, Wannapawn Watsuntorn, Zhongming Wang, Nuttha Chuengcharoenphanich, Warawut Chulalaksananukul, Cheryanus Butkumchote, Chompunuch Glinwong, Poompat Niltaya, Wei Qi |
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| Rok vydání: | 2020 |
| Předmět: |
Environmental Engineering
ASTM D6751 Health Toxicology and Mutagenesis 0208 environmental biotechnology Saccharomyces cerevisiae 02 engineering and technology 010501 environmental sciences 01 natural sciences Environmental Chemistry Food science Biomass 0105 earth and related environmental sciences Biodiesel biology Chemistry Fatty Acids Public Health Environmental and Occupational Health food and beverages EN 14214 General Medicine General Chemistry Transesterification biology.organism_classification Thailand Pollution Lipids Yeast 020801 environmental engineering Biodiesel production Yield (chemistry) Biofuels lipids (amino acids peptides and proteins) Copper |
| Zdroj: | Chemosphere. 280 |
| ISSN: | 1879-1298 |
| Popis: | This study reports on the novel Saccharomyces cerevisiae CU-TPD4 that was isolated from coconut waste residues obtained from a coconut factory in Thailand. The CU-TPD4 isolate was confirmed to be a S. cerevisiae by molecular analysis and to be an oleaginous yeast with more than 20% (w/w) of the cell dry weight (CDW) present in the form of lipids. The lipid content and lipid yield of CU-TPD4 (52.96 ± 1.15% of CDW and 1.78 ± 0.06 g/L, respectively) under optimized growth conditions were much higher than those under normal growth conditions (22.65 ± 1.32% of CDW and 1.24 ± 0.12 g/L, respectively). The major fatty acids produced by CU-TPD4 were oleic (C18:1), palmitoleic (C16:1), stearic (C18:0), and palmitic (C16:0) acids. Mathematical estimation of the physical properties of the biodiesel obtained by transesterification of the extracted lipid suggested it was suitable as biodiesel with respect to the ASTM D6751 and EN 14214 international standards. Consequently, S. cerevisiae CU-TPD4 is expected to emerge as a promising alternative for biodiesel production. |
| Databáze: | OpenAIRE |
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