Effect of Different Methods of Trypsinization on Cell Viability and Clinical Outcome in Vitiligo Patients Undergoing Noncultured Epidermal Cellular Suspension
| Autor: | Heba I. Gawdat, Rehab A. Hegazy, Nesrin S Elkhouly, Dalia M Bassiouny, Sally Doss, Hoda Rasheed, Davinder Parsad, Samia Esmat |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Cell Survival Urology Vitiligo Skin Pigmentation Dermatology Cell Separation Transplantation Autologous law.invention 030207 dermatology & venereal diseases 03 medical and health sciences Young Adult 0302 clinical medicine Randomized controlled trial law Biopsy medicine Photography Humans Trypsin Viability assay Prospective Studies Prospective cohort study Skin medicine.diagnostic_test business.industry Temperature General Medicine Middle Aged medicine.disease Combined Modality Therapy Trypsinization Transplantation Treatment Outcome Multicenter study Epidermal Cells Patient Satisfaction 030220 oncology & carcinogenesis Surgery Female Ultraviolet Therapy business Follow-Up Studies |
| Zdroj: | Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.]. 46(10) |
| ISSN: | 1524-4725 |
| Popis: | Background Noncultured Epidermal Cell Suspension (NCECS) is a surgical modality used in treating stable vitiligo. Trypsinization of the epidermis may be done either at 4°C overnight (cold) or at 37°C for 30 to 50 minutes (warm). Recently, trypsinization was done at room temperature (25°C) in an in vitro trial. Objective To compare different trypsinization techniques in NCECS regarding cell viability and clinical outcome. Methods This comparative multicenter study was conducted on 20 patients with stable nonsegmental vitiligo. In each patient, 3, nonacral vitiligo lesions were randomly assigned for treatment by NCECS prepared by warm, room temperature, and cold trypsinization techniques, respectively. A perilesional biopsy was taken from each of the 3 treated lesions as an objective measure of disease stability. After transplantation, all patients received narrow-band ultraviolet B twice weekly for 6 months. Cell viability was assessed in each technique, as well as clinical outcome in all treated lesions. Results Warm and room temperature trypsinization techniques were comparable with each other. Both were significantly better than the cold technique regarding viability and repigmentation. Conclusion Room temperature trypsinization can be used as a convenient substitute to warm trypsinization. Cold trypsinization is not recommended because of its poor results and poor patient satisfaction. |
| Databáze: | OpenAIRE |
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