The Osteogenic Function of Danggui Buxue Tang, a Herbal Decoction Containing Astragali Radix and Angelicae Sinensis Radix, Is Optimized by Boiling the Two Herbs Together: Signaling Analyses Revealed by Systems Biology
| Autor: | Karl Wah Keung Tsim, Anna X D Yu, Henry H. L. Lam, Tina T. X. Dong, Tin Yan Wong, Kenneth Kin Leung Kwan |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Systems biology Energy metabolism Bioengineering Decoction Traditional Chinese medicine TP1-1185 complex mixtures 03 medical and health sciences 0302 clinical medicine bone differentiation Chemical Engineering (miscellaneous) Radix QD1-999 mass spectrometry mesenchymal stem cells Danggui Buxue Tang Traditional medicine Chemistry Process Chemistry and Technology Chemical technology food and beverages Lipid metabolism multi-omics 030104 developmental biology 030220 oncology & carcinogenesis boiling process Function (biology) |
| Zdroj: | Processes Volume 9 Issue 7 Processes, Vol 9, Iss 1119, p 1119 (2021) |
| ISSN: | 2227-9717 |
| DOI: | 10.3390/pr9071119 |
| Popis: | The therapeutic efficacy of a herbal mixture, being multi-target, multi-function and multi-pathway, is the niche of traditional Chinese medicine (TCM). Systems biology can dissect the network of signaling mechanisms in a complex biological system. In preparing TCM decoctions, the boiling of herbs together in water is a common practice however, the rationale of this specific preparation has not been fully revealed. An approach of mass-spectrometry-based multi-omics was employed to examine the profiles of the cellular pathways, so as to understand the pharmacological efficacy of Danggui Buxue Tang (DBT), a Chinese herbal mixture containing Astragali Radix and Angelicae Sinensis Radix, in cultured rat osteoblasts and mesenchymal stem cells. The results, generated from omics analyses, were compared from DBT-treated osteoblasts to those of treating the herbal extract by simple mixing of extracts from Astragali Radix and Angelicae Sinensis Radix, i.e., herbal mixture without boiling together. The signaling pathways responsible for energy metabolism and amino acid metabolism showed distinct activation, as triggered by DBT, in contrast to simple mixing of two herbal extracts. The result supports that boiling the herbs together is designed to maximize the osteoblastic function of DBT, such as in energy and lipid metabolism. This harmony of TCM formulation, by having interactive functions of two herbs during preparation, is being illustrated. The systems biology approach provides new and essential insights into the synergy of herbal preparation. Well-defined multiple targets and multiple pathways in different levels of omics are the key to modernizing TCM. |
| Databáze: | OpenAIRE |
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