Enhancement of the immune response and protection against Vibrio parahaemolyticus by indigenous probiotic Bacillus strains in mud crab (Scylla paramamosain)
Autor: | Chuanbiao Li, Zhong Hu, Wu Huijuan, Zhongzhen Li, Xiaobo Wen, Shengkang Li, Zhao Zhang, Yueling Zhang, Sun Lingbin |
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Rok vydání: | 2014 |
Předmět: |
animal structures
Genotype Brachyura Bacillus cereus Scylla paramamosain Bacillus Bacillus subtilis Aquatic Science law.invention Microbiology Probiotic law Environmental Chemistry Animals Bile DNA Primers Respiratory Burst Analysis of Variance biology Bacillus pumilus Vibrio parahaemolyticus Microbiota Probiotics food and beverages General Medicine Hydrogen-Ion Concentration biology.organism_classification 16S ribosomal RNA Culture Media body regions Gastrointestinal Tract Hydrophobic and Hydrophilic Interactions |
Zdroj: | Fishshellfish immunology. 41(2) |
ISSN: | 1095-9947 |
Popis: | In a previous study, bacterial communities of the intestine in three populations of crabs (wild crabs, pond-raised healthy crabs and diseased crabs) were probed by culture-independent methods. In this study, we examined the intestinal communities of the crabs by bacterial cultivation with a variety of media. A total of 135 bacterial strains were isolated from three populations of mud crabs. The strains were screened for antagonistic activity against Vibrio parahaemolyticus using an agar spot assay. Antagonistic strains were then identified by 16S rRNA gene sequence analysis. Three strains (Bacillus subtilis DCU, Bacillus pumilus BP, Bacillus cereus HL7) with the strongest antagonistic activity were further evaluated for their probiotic characteristics. The results showed that two (BP and DCU) of them were able to survive low pH and high bile concentrations, showed good adherence characteristics and a broad spectrum of antibiotic resistance. The probiotic effects were then tested by feeding juvenile mud crabs (Scylla paramamosain) with foods supplemented with 10(5) CFU/g of BP or DCU for 30 days before being subjected to an immersion challenge with V. parahaemolyticus for 48 h. The treated crabs showed significantly higher expression levels of immune related genes (CAT, proPO and SOD) and activities of respiratory burst than that in controlled groups. Crabs treated with BP and DCU supplemented diets exhibited survival rates of 76.67% and 78.33%, respectively, whereas survival rate was 54.88% in crabs not treated with the probiotics. The data showed that indigenous mud-associated microbiota, such as DCU and BP, have potential application in controlling pathogenic Vibriosis in mud crab aquaculture. |
Databáze: | OpenAIRE |
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