Novel Lentivirus-Based Method for Rapid Selection of Inhibitory Nanobody against PRRSV
Autor: | Kai-Xia Lei, Guangwei Han, Huiling Xu, Fang He, Ze-Hui Liu |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Swine viruses animal diseases 030106 microbiology lcsh:QR1-502 Virus Replication Immunofluorescence Antiviral Agents Article lcsh:Microbiology Cell Line 03 medical and health sciences Western blot Peptide Library Virology medicine Animals Porcine respiratory and reproductive syndrome virus antiviral strategy biology medicine.diagnostic_test Lentivirus virus diseases Single-Domain Antibodies Porcine reproductive and respiratory syndrome virus biology.organism_classification lentivirus based nanobody library 030104 developmental biology Infectious Diseases PRRSV nanobody selection Camelids New World Function (biology) Alternative strategy |
Zdroj: | Viruses Volume 12 Issue 2 Viruses, Vol 12, Iss 2, p 229 (2020) |
ISSN: | 1999-4915 |
DOI: | 10.3390/v12020229 |
Popis: | The emergence and re-emergence of porcine reproductive and respiratory syndrome virus (PRRSV) has resulted in huge economic losses for the swine industry. Current vaccines are of limited efficacy against endemic circulating PRRSV variants. New strategies against PRRSV infection are in urgent need. Here, a nanobody library in Marc-145 cells is constructed for antiviral nanobodies. Nanobody encoding sequences from two non-immunized llamas were cloned to generate a pseudotyped lentiviral library. Several candidates were selected from survival cells post-PRRSV inoculation and further characterized. Nb9 was identified with strong antiviral activity. Moreover, Nb9 exerted antiviral activity via its interaction with PRRSV viral proteins, as revealed by immunofluorescence assay and Western blot. Taken together, the novel function-based screen of the lentivirus nanobody library, instead of the conventional affinity-based screen, offers an alternative strategy for antiviral reagents against PRRSV and other pathogens. |
Databáze: | OpenAIRE |
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