Adipose Tissue-Derived Mesenchymal Stem Cells Exert In Vitro Immunomodulatory and Beta Cell Protective Functions in Streptozotocin-Induced Diabetic Mice Model

Autor: Jamal Mohammadi, Masoud Soleimani, Nader Tajik, Hossein Rahavi, Seyed Mahmoud Hashemi
Jazyk: angličtina
Rok vydání: 2015
Předmět:
medicine.medical_specialty
Article Subject
endocrine system diseases
Endocrinology
Diabetes and Metabolism
medicine.medical_treatment
Islets of Langerhans Transplantation
Adipose tissue
Biology
lcsh:Diseases of the endocrine glands. Clinical endocrinology
Streptozocin
Proinflammatory cytokine
Diabetes Mellitus
Experimental
Immunophenotyping
Islets of Langerhans
Mice
Endocrinology
Internal medicine
Insulin-Secreting Cells
Insulin Secretion
medicine
Animals
Insulin
Cell Lineage
Cell Proliferation
Inflammation
geography
geography.geographical_feature_category
lcsh:RC648-665
Pancreatic islets
Mesenchymal stem cell
Mesenchymal Stem Cells
Islet
Streptozotocin
Mice
Inbred C57BL
medicine.anatomical_structure
Cytokine
Diabetes Mellitus
Type 1
Adipose Tissue
Cytokines
Female
Beta cell
Spleen
medicine.drug
Research Article
Zdroj: Journal of Diabetes Research
Journal of Diabetes Research, Vol 2015 (2015)
ISSN: 2314-6753
2314-6745
Popis: Regenerative and immunomodulatory properties of mesenchymal stem cells (MSCs) might be applied for type 1 diabetes mellitus (T1DM) treatment. Thus, we proposed in vitro assessment of adipose tissue-derived MSCs (AT-MSCs) immunomodulation on autoimmune response along with beta cell protection in streptozotocin- (STZ-) induced diabetic C57BL/6 mice model. MSCs were extracted from abdominal adipose tissue of normal mice and cultured to proliferate. Diabetic mice were prepared by administration of multiple low-doses of streptozotocin. Pancreatic islets were isolated from normal mice and splenocytes prepared from normal and diabetic mice. Proliferation, cytokine production, and insulin secretion assays were performed in coculture experiments. AT-MSCs inhibited splenocytes proliferative response to specific (islet lysate) and nonspecific (PHA) triggers in a dose-dependent manner (P<0.05). Decreased production of proinflammatory cytokines, such as IFN-γ, IL-2, and IL-17, and increased secretion of regulatory cytokines such as TGF-β, IL-4, IL-10, and IL-13 by stimulated splenocytes were also shown in response to islet lysate or PHA stimulants (P<0.05). Finally, we demonstrated that AT-MSCs could effectively sustain viability as well as insulin secretion potential of pancreatic islets in the presence of reactive splenocytes (P<0.05). In conclusion, it seems that MSCs may provide a new horizon for T1DM cell therapy and islet transplantation in the future.
Databáze: OpenAIRE
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