Prostaglandin-Like Substances Appearing in Canine Renal Venous Blood During Renal Ischemia
| Autor: | J. C. McGiff, Norberto A. Terragno, Kevin K. F. Ng, James C. Strand, Andrew J. Lonigro, Mary Anne Williamson, James B. Lee, Keith Crowshaw |
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| Rok vydání: | 1970 |
| Předmět: |
Male
medicine.medical_specialty Physiology Prostaglandin Kidney Renal Artery Obstruction Renal Veins chemistry.chemical_compound Dogs Ischemia Internal medicine Methods medicine Renal medulla Animals Antihypertensive Agents Renal ischemia business.industry Biological activity Venous blood Angiotensin II Rats Surgery Perfusion Endocrinology medicine.anatomical_structure chemistry Renal blood flow Prostaglandins Chromatography Thin Layer Cardiology and Cardiovascular Medicine business Chickens |
| Zdroj: | Circulation Research. 27:765-782 |
| ISSN: | 1524-4571 0009-7330 |
| DOI: | 10.1161/01.res.27.5.765 |
| Popis: | Renal prostaglandins (PC s ) might mediate an antihypertensive function of the kidney. The blood-superfused organ technique possesses the sensitivity (threshold < 0.4 ng/ml blood) and specificity required for identification of PGs in blood. Induction of unilateral renal ischemia in 14 chloraloseanesthetized dogs reduced renal blood flows from a mean value of 257 to 109 ml/min on the ischemic side and from 250 to 209 ml/min on the contralateral side. Concomitantly, PG-like substances were detected by assay organs in the venous blood of ischemic (13 experiments) and contralateral (11 experiments) kidneys. In one experiment, in a spontaneously hypertensive dog, PGs were not detected during renal ischemia. Renal venous blood and renal medullary tissue were extracted for acidic lipids and assayed for PG-like substances. Extracts of venous blood collected during renal ischemia and extracts of renal medulla yielded substances with biological activity indistinguishable from PG-like substances or PG standards. Chromatographic characterization of PG-like substances suggests that they are predominantly a mixture of PGE 2 and PGF 2α . |
| Databáze: | OpenAIRE |
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