Calcium entry is regulated by Zn2+ in relation to extracellular ionic environment in human airway epithelial cells
| Autor: | László Csernoch, Ágnes Pataki, Ákos Zsembery, Gyula Peter Szigeti, Péter Várnai, Tamas Danko, Márta Füzi, Gábor Raffai, Dóra Hargitai |
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| Rok vydání: | 2009 |
| Předmět: |
Pulmonary and Respiratory Medicine
Physiology Sodium chemistry.chemical_element Cystic Fibrosis Transmembrane Conductance Regulator Suramin Calcium Transfection Divalent Lactones Adenosine Triphosphate Hexokinase Extracellular Humans Calcium Signaling Estrenes Receptor Cell Line Transformed Calcium metabolism chemistry.chemical_classification Chemistry Receptors Purinergic P2 General Neuroscience Purinergic receptor Apyrase Epithelial Cells Extracellular Fluid Pyrrolidinones Zinc Biochemistry Gene Expression Regulation Cell culture Receptors Purinergic P2X Biophysics Sesquiterpenes Gene Deletion Platelet Aggregation Inhibitors |
| Zdroj: | Respiratory physiologyneurobiology. 170(1) |
| ISSN: | 1878-1519 |
| Popis: | The extracellular pH, sodium and divalent cation concentrations influence the ATP-induced changes in cytosolic Ca(2+) concentration ([Ca(2+)](i)). This elevation of [Ca(2+)](i) and activation of Ca(2+)-dependent Cl(-) channels represent a possible therapeutic approach in cystic fibrosis (CF). We investigated the changes of [Ca(2+)](i) in different external ionic environment, and P2X purinergic receptors (P2XRs) expression in the control and CF airway epithelial cells. The parallel removal of Na(+) and alkalinization of the extracellular solution increased the amplitude of sustained ATP-induced Ca(2+) signals independent of wild-type or mutant CFTR expression. The ATP-induced Ca(2+) entry was either inhibited or stimulated by Zn(2+) depending on the extracellular Na(+) concentration. In Na(+)-free environment, Zn(2+) and other divalent cations elicited a biphasic Ca(2+) signal. Immunohistochemical data suggest that, multiple subtypes of P2XRs are expressed in these airway epithelial cells. In conclusion, Ca(2+) entry is finely regulated by external ionic environment. Therefore, we speculate that properly compiled aerosols could influence efficacy of zinc-based therapy in CF. |
| Databáze: | OpenAIRE |
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