Dynamical release nanospheres containing cell growth factor from biopolymer hydrogel via reversible covalent conjugation
| Autor: | Yang Jia, Zhilin Yan, Yongjie Xu, Zhonghua Ling, Ye Ma, Bowen Ren, Yong Chen, Huaping Tan, Shoukang Du, Saibo Qian, Xueyun Chen, Jianliang Li, Xiaohong Hu |
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| Rok vydání: | 2018 |
| Předmět: |
Scaffold
Cell Survival Surface Properties Drug Compounding Biomedical Engineering Biophysics Bioengineering 02 engineering and technology engineering.material 010402 general chemistry 01 natural sciences Biomaterials Biopolymers Tissue engineering Humans Insulin-Like Growth Factor I Particle Size Maltose Cell Proliferation Drug Carriers Tissue Engineering Tissue Scaffolds Cell growth Chemistry Regeneration (biology) Hydrogels Mesenchymal Stem Cells 021001 nanoscience & nanotechnology Boronic Acids 0104 chemical sciences Drug Liberation Kinetics Cross-Linking Reagents Glucose Adipose Tissue Cell culture Self-healing hydrogels Drug delivery engineering Biopolymer 0210 nano-technology Nanospheres |
| Zdroj: | Journal of biomaterials science. Polymer edition. 29(11) |
| ISSN: | 1568-5624 |
| Popis: | For practical adipose regeneration, the challenge is to dynamically deliver the key adipogenic insulin-like growth factors in hydrogels to induce adipogenesis. In order to achieve dynamic release, smart hydrogels to sense the change in the blood glucose concentration is required when glucose concentration increases. In this study, a heparin-based hydrogel has been developed for use in dynamic delivery of heparin nanospheres containing insulin-like growth factor. The gel scaffold was facilely prepared in physiological conditions by the formation of boronate-maltose ester cross-links between boronate and maltose groups of heparin derivatives. Due to its intrinsic glucose-sensitivity, the exposure of gel scaffold to glucose induces maltose functionalized nanospheres dissociation off hydrogel network and thereby could dynamically move into the microenvironment. The potential of the hydrogel as a cell scaffold was demonstrated by encapsulation of human adipose-derived stem cells (ASCs) within the gel matrix in vitro. Cell culture showed that this dynamic hydrogel could support survival and proliferation of ASCs. This biocompatible coupling chemistry has the advantage that it introduces no potentially cytotoxic groups into injectable gel scaffolds formed and can create a more biomimetic microenvironment for drug and cell delivery, rendering them more suitable for potential in vivo biomedical applications. All these results indicate that this biocompatible gel scaffold can render the formulation of a therapeutically effective platform for diabetes treatment and adipose regeneration. |
| Databáze: | OpenAIRE |
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