Cross-species hybridization of microarrays for studying tumor transcriptome of brain metastasis
| Autor: | Gordon B. Mills, Sun Jin Kim, Isaiah J. Fidler, Ju Seog Lee, Sang Bae Kim, Se Lyun Yoon, Soo Mi Kim, Yun Yong Park, Eun Sung Park, Hyun Goo Woo, Seung Wook Kim, Sun Hee Leem, Jae Ho Cheong |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Stromal cell Transplantation Heterologous Mice Nude Biology Epigenesis Genetic Metastasis Transcriptome Mice Species Specificity Cancer stem cell Cell Line Tumor Tumor Microenvironment medicine Animals Humans DNA Primers Oligonucleotide Array Sequence Analysis Neurons Mice Inbred BALB C Tumor microenvironment Multidisciplinary Base Sequence Brain Neoplasms Nucleic Acid Hybridization Cancer DNA Neoplasm DNA Methylation Biological Sciences medicine.disease Molecular biology Coculture Techniques Cell biology Astrocytes Cancer cell Reprogramming Neoplasm Transplantation |
| Zdroj: | Proceedings of the National Academy of Sciences. 108:17456-17461 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Although the importance of the cellular microenvironment (soil) during invasion and metastasis of cancer cells (seed) has been well-recognized, technical challenges have limited the ability to assess the influence of the microenvironment on cancer cells at the molecular level. Here, we show that an experimental strategy, competitive cross-species hybridization of microarray experiments, can characterize the influence of different microenvironments on cancer cells by independently extracting gene expression data of cancer and host cells when human cancer cells were xenografted into different organ sites of immunocompromised mice. Surprisingly, the analysis of gene expression data showed that the brain microenvironment induces complete reprogramming of metastasized cancer cells, resulting in a gain of neuronal cell characteristics and mimicking neurogenesis during development. We also show that epigenetic changes coincide with transcriptional reprogramming in cancer cells. These observations provide proof of principle for competitive cross-species hybridization of microarray experiments to characterize the effect of the microenvironment on tumor cell behavior. |
| Databáze: | OpenAIRE |
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