Interleukin-18 expression and modulation in human corneal epithelial cells
| Autor: | J B Harten, S W Caughman, Hans E. Grossniklaus, Shubhada M. Naik, Cheryl A. Armstrong, Guido J. Burbach, Stefan Dithmar, Sherry L. Ward, L Liu, John C. Ansel |
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| Rok vydání: | 2002 |
| Předmět: |
Lipopolysaccharides
medicine.medical_treatment Enzyme-Linked Immunosorbent Assay Biology Cell Line Immunoenzyme Techniques Cellular and Molecular Neuroscience chemistry.chemical_compound Polydeoxyribonucleotides Interferon Cornea medicine Humans Northern blot RNA Messenger Epithelium Corneal Interleukin-18 Blotting Northern Molecular biology Sensory Systems Blot Ophthalmology medicine.anatomical_structure Cytokine chemistry Gene Expression Regulation Cell culture Immunology Phorbol Tetradecanoylphorbol Acetate Interleukin 18 medicine.drug |
| Zdroj: | Current eye research. 23(1) |
| ISSN: | 0271-3683 |
| Popis: | The interferon-gamma-inducing factor Interleukin-18 (IL-18) is a recently described cytokine that appears to have multiple important pro-inflammatory effects including the induction of interferon-gamma (IFN-gamma) by activated T-cells. The expression of IL-18 by human cornea has not been previously reported. In the present study, we examine the possibility that human corneal epithelial cells are capable of producing this leukocyte-activating factor which may play an important role in IFN-gamma-dependent inflammation responses in the cornea.Northern blot analysis and ELISA were used to investigate the in vitro expression of IL-18 mRNA and protein respectively in primary (HCEC) and transformed human corneal epithelial cells (HCET). To determine if IL-18 expression was modulated by pro-inflammatory mediators, cells were treated with lipopolysaccharide (LPS), phorbol 12-myristate 13-acetate (PMA) or synthetic double stranded RNA (poly dI : dC). IL-18 bioactivity was determined in a leukocyte interferon-gamma induction assay and IL-18 was immunolocalized in whole human cornea by immunohistochemistry using a specific anti-IL-18 antibody.IL-18 mRNA and bioactive protein was constitutively expressed by human corneal epithelial cells and upregulated by PMA, LPS and poly dI : dC. The constitutive expression of IL-18 protein immunoreactivity was also demonstrated in the epithelial cells of whole human cornea tissue.This is the first study demonstrating that corneal epithelial cells are capable of producing the IFN-gamma inducing factor IL-18. Increased bioactive corneal IL-18 production can be induced by a number of pro-inflammatory agents and may play an important role in initiating gamma-interferon-mediated inflammatory responses in the cornea. |
| Databáze: | OpenAIRE |
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