Properties of Yersinia entetocolitica and Yersinia pseudotuberculosis in red blood cell concentrate of different ABO groups during 30-day storage at 4 °C
| Autor: | T. Radoucheva, Nadya Markova, P. Orozova |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Microbiology (medical)
Blood Bactericidal Activity Erythrocytes Time Factors growth Blood Donors Yersinia Bacterial growth Y. enterocolitica ABO Blood-Group System Microbiology Blood product ABO blood group system medicine Humans Yersinia pseudotuberculosis Yersinia enterocolitica Incubation Y. pseudotuberculosis biology susceptibility to serum Temperature General Medicine ABO blood groups biology.organism_classification Red blood cell Infectious Diseases medicine.anatomical_structure Blood Preservation Glycolysis |
| Zdroj: | Clinical Microbiology and Infection. 7:358-361 |
| ISSN: | 1198-743X |
| DOI: | 10.1046/j.1198-743x.2001.00277.x |
| Popis: | Objective The present study aimed to investigate the psychrophilic properties of Yersinia enterocolitica and Yersinia pseudotuberculosis as contaminants of donated blood. Methods Bags with red blood cell concentrates (RBCCs) of A, B, and AB blood groups were inoculated with a bacterial suspension of Y. enterocolitica (0 : 3 and 0 : 8) and Y. pseudotuberculosis (serovars I and III) and stored at 4 °C for 30 days. Bacterial growth was measured at selected intervals after inoculation. Initial strains and their subcultures (isolated after 30 days' incubation at 4 °C) were tested for glycolytic activity and susceptibility to the bactericidal action of human serum. Results It was found that all strains grew well in the RBCCs of A, B, and AB groups. After incubation at 4 °C they increased their glycolytic activity and became more sensitive to the killing ability of human serum. Conclusions The prolonged storage of contaminated Y. enterocolitica and Y. pseudotuberculosis RBCCs at 4 °C induces bacterial multiplication to high levels and stimulates glycolytic activity of bacterial cells. |
| Databáze: | OpenAIRE |
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