Simultaneous determination of felbamate and four metabolites in rat cerebrospinal fluid by high-performance liquid chromatography
| Autor: | R.D. Sofia, N. Kucharczyk, L.A. Romanyshyn, J. K. Wichmann |
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| Rok vydání: | 1993 |
| Předmět: |
Chromatography
Elution Metabolite Phenylcarbamates General Chemistry Ultraviolet absorbance Phosphate High-performance liquid chromatography Felbamate Rats chemistry.chemical_compound Cerebrospinal fluid chemistry Propylene Glycols medicine Animals Anticonvulsants Spectrophotometry Ultraviolet Quantitative analysis (chemistry) Biotransformation Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of chromatography. 622(2) |
| Popis: | An isocratic liquid chromatographic method for direct sample injection has been developed for the quantitation of felbamate and four metabolites in rat cerebrospinal fluid. The method uses 0.050- or 0.025-ml aliquots of cerebrospinal fluid diluted with equal volumes of internal standard. Chromatography is performed on a 150 mm x 4.6 mm I.D. Spherisorb ODS2, 3-microns HPLC column eluted with a phosphate buffer-acetonitrile-methanol (820:120:60, v/v/v) mobile phase and ultraviolet absorbance detection at 210 nm. The linear quantitation ranges are: felbamate and the 2-hydroxy metabolite 0.195-200 micrograms/ml, the propionic acid metabolite 0.195-50.0 micrograms/ml, the p-hydroxy metabolite 0.781 to 50.0 micrograms/ml, and the monocarbamate metabolite 0.098-50.0 micrograms/ml. |
| Databáze: | OpenAIRE |
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