Increased Dickkopf-1 expression in breast cancer bone metastases
Autor: | Patrick Garnero, Delphine Goehrig, Fabrice Journé, Philippe Clézardin, Jean-Jacques Body, Nathalie Voorzanger-Rousselot, Valérie Doriath |
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Rok vydání: | 2007 |
Předmět: |
Bone turnover
Cancer Research Pathology medicine.medical_specialty bone turnover Osteolysis Blotting Western Mice Nude Bone Neoplasms Breast Neoplasms Enzyme-Linked Immunosorbent Assay Bone resorption Bone remodeling Dickkopf-1 Mice Breast cancer breast cancer Tumor Cells Cultured medicine Animals Humans RNA Messenger RNA Neoplasm Bone Resorption skin and connective tissue diseases Molecular Diagnostics Wnt signalling bone metastasis Reverse Transcriptase Polymerase Chain Reaction business.industry Bone metastasis Cancer Sciences bio-médicales et agricoles medicine.disease medicine.anatomical_structure Oncology Cancer cell Intercellular Signaling Peptides and Proteins Female Bone marrow business |
Zdroj: | British Journal of Cancer British Journal of Cancer, 97 (7 |
ISSN: | 1532-1827 0007-0920 |
Popis: | The aim of this study was to determine whether Dickkopf-1 (Dkk-1) expression in breast cancer was associated with bone metastases. We first analysed Dkk-1 expression by human breast cancer cell lines that induce osteolytic or osteoblastic lesions in animals. Dickkopf-1 levels were then measured in the bone marrow aspirates of hind limbs from eight NMRI mice inoculated with breast cancer cells that induced bone metastases and 11 age-matched non-inoculated control animals. Finally, Dkk-1 was measured in the serum of 17 women with breast cancer in complete remission, 19 women with breast cancer and bone metastases, 16 women with breast cancer and metastases at non-bone sites and 16 healthy women. Only breast cancer cells that induce osteolytic lesions in animals produced Dkk-1. There was a six-fold increase in Dkk-1 levels in the bone marrow from animals inoculated with MDA-B02 cells when compared with that of control non-inoculated animals (P=0.003). Median Dkk-1 levels in the serum of patients with breast cancer and bone metastases were significantly higher than levels of patients in complete remission (P=0.016), patients with breast cancer having metastases at non-bone sites (P SCOPUS: ar.j info:eu-repo/semantics/published |
Databáze: | OpenAIRE |
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