Cold-regulated protein (SlCOR413IM1) confers chilling stress tolerance in tomato plants
| Autor: | Xinchun Dong, Qing-Wei Meng, Chong Chen, Xiaocui Ma, Wei Lv, Minmin Yang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Photosystem II Physiology Transgene Acclimatization Plant Science Photosynthesis 01 natural sciences Chloroplast membrane 03 medical and health sciences Solanum lycopersicum Gene Expression Regulation Plant Sense (molecular biology) Genetics Glyceraldehyde 3-phosphate dehydrogenase Plant Proteins biology Chemistry Cold-Shock Response fungi food and beverages Photosynthetic capacity Chloroplast Horticulture 030104 developmental biology biology.protein Cold Shock Proteins and Peptides 010606 plant biology & botany |
| Zdroj: | Plant physiology and biochemistry : PPB. 124 |
| ISSN: | 1873-2690 |
| Popis: | Chilling stress severely affects the growth, development and productivity of crops. Chloroplast, a photosynthesis site, is extremely sensitive to chilling stress. In this study, the functions of a gene encoding a cold-regulated protein (SlCOR413IM1) under chilling stress were investigated using sense and antisense transgenic tomatoes. Under chilling stress, SlCOR413IM1 expression was rapidly induced and the sense lines exhibited better growth state of seedlings and grown tomato plants. Overexpression of SlCOR413IM1 alleviated chilling-induced damage to the chloroplast membrane and structure, whereas suppression of SlCOR413IM1 aggravated the damage to chloroplast. Moreover, the net photosynthetic rate (Pn), maximum photochemical efficiency of photosystem II (PSII) (Fv/Fm), actual photochemical efficiency of PSII (ΦPSII) and the activities of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and stromal fructose-1, 6-bisphosphatase (sFBPase) were higher in the sense lines than those in the antisense lines. Hence, the inhibition of photosynthetic capacity was less severe in the sense lines but more severe in the antisense lines compared with that in wild-type (WT) plants. Taken together, overexpression of SlCOR413IM1 enhanced the chilling stress tolerance, whereas suppression of this gene increased the chilling sensitivity of tomato plants. |
| Databáze: | OpenAIRE |
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