Mitochondrial Patch Clamp of Beige Adipocytes Reveals UCP1-Positive and UCP1-Negative Cells Both Exhibiting Futile Creatine Cycling
| Autor: | Marta G. Bogaczynska, Lawrence Kazak, Bruce M. Spiegelman, Ambre M. Bertholet, Gabrielle L. Wainwright, Edward T. Chouchani, Ishan Paranjpe, Shingo Kajimura, Alexandre Betourne, Yuriy Kirichok |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male 0106 biological sciences Purine Patch-Clamp Techniques Physiology Adipose tissue White adipose tissue 01 natural sciences Biochemistry Oxidative Phosphorylation Ion Channels Mice chemistry.chemical_compound 0302 clinical medicine Adipose Tissue Brown Adipocytes Browning Nucleotide Adipocytes Beige Receptor Inner mitochondrial membrane Purine Nucleotides Uncoupling Protein 1 Epididymis chemistry.chemical_classification 0303 health sciences Adipogenesis Chemistry Fatty Acids Thermogenesis Thermogenin Mitochondria Adipose Tissue Protons medicine.medical_specialty Cell Respiration Biophysics Inguinal Canal Biology Creatine Article Mitochondrial Proteins 03 medical and health sciences Internal medicine medicine Animals Humans Obesity Patch clamp Molecular Biology 030304 developmental biology Cell Biology 030104 developmental biology Endocrinology Receptors Adrenergic beta-3 030217 neurology & neurosurgery 010606 plant biology & botany |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1859:e37 |
| ISSN: | 0005-2728 |
| Popis: | Summary Cold and other environmental factors induce "browning" of white fat depots—development of beige adipocytes with morphological and functional resemblance to brown fat. Similar to brown fat, beige adipocytes are assumed to express mitochondrial uncoupling protein 1 (UCP1) and are thermogenic due to the UCP1-mediated H + leak across the inner mitochondrial membrane. However, this assumption has never been tested directly. Herein we patch clamped the inner mitochondrial membrane of beige and brown fat to provide a direct comparison of their thermogenic H + leak ( I H ). All inguinal beige adipocytes had robust UCP1-dependent I H comparable to brown fat, but it was about three times less sensitive to purine nucleotide inhibition. Strikingly, only ∼15% of epididymal beige adipocytes had I H , while in the rest UCP1-dependent I H was undetectable. Despite the absence of UCP1 in the majority of epididymal beige adipocytes, these cells employ prominent creatine cycling as a UCP1-independent thermogenic mechanism. |
| Databáze: | OpenAIRE |
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