Biophysical Properties of Frog Virus and Its Deoxyribonucleic Acid: Fate of Radioactive Virus in the Early Stage of Infection
Autor: | William R. Smith, Brian R. McAuslan |
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Rok vydání: | 1969 |
Předmět: |
Potassium tartrate
Guanine viruses Immunology In Vitro Techniques Biology Kidney Tritium Microbiology Chloride Virus Cell Line Viral Proteins chemistry.chemical_compound Cricetinae Virology Animal Viruses Sodium citrate Centrifugation Density Gradient medicine Animals Magnesium Herpesviridae Differential centrifugation Infectivity Carbon Isotopes Antibiotics Antineoplastic Molecular biology Sedimentation coefficient Microscopy Electron Biochemistry chemistry Insect Science DNA Viral Viruses Unclassified Adsorption Anura Thymidine medicine.drug |
Zdroj: | Journal of Virology. 4:339-347 |
ISSN: | 1098-5514 0022-538X |
DOI: | 10.1128/jvi.4.4.339-347.1969 |
Popis: | Frog virus (FV-3) was banded by isopycnic centrifugation in cesium chloride, sucrose, or potassium tartrate. Two bands of infectivity were regularly found at positions in cesium chloride corresponding to densities of 1.26 and 1.30 g/cm 3 , respectively. Deoxyribonucleic acid from either band had the following characteristics: double-stranded; a T m of 76.3 C in 0.1 SSC (0.015 m NaCl plus 0.015 m sodium citrate) and a buoyant density of 1.720 g/cm 3 in cesium chloride, corresponding to a guanine plus cytosine content of 56 to 58% and a molecular weight of 130 × 10 6 daltons, determined by velocity sedimentation. These data, together with electron micrographs of sections of cells infected with material from either band suggest that two types of infectious frog virus particles exists, rather than a second virus in the frog virus stocks. The composition of frog virus was determined. It was found that highly purified preparations of frog virus were composed of 55.8% protein, 30.1% deoxyribonucleic acid, and 14.2% lipid. The kinetics of adsorption and uncoating of FV-3 was studied with radioactive virus. Uncoating is comparatively rapid and in contrast to poxvirus is unaffected by inhibitors of protein synthesis. |
Databáze: | OpenAIRE |
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