Analyzing somatosensory axon projections with the sensory neuron-specific Advillin gene
| Autor: | Sara Abbott, Fan Wang, Yi Qi, Bao-Xia Han, Hiroshi Hasegawa |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Hindbrain
Sensory system Mice Transgenic Biology Somatosensory system Electron Transport Complex IV Mice Stimulus modality Neural Pathways medicine Animals Neurons Afferent Peripheral Nerves Axon Cells Cultured Skin Neuronal Plasticity General Neuroscience Microfilament Proteins Gene Expression Regulation Developmental Articles Spinal cord Alkaline Phosphatase Embryo Mammalian Sensory neuron Axons Nerve Regeneration medicine.anatomical_structure nervous system Animals Newborn Spinal Cord Trigeminal Ganglion Vibrissae Brainstem Neuroscience Brain Stem |
| Zdroj: | The Journal of neuroscience : the official journal of the Society for Neuroscience. 27(52) |
| ISSN: | 1529-2401 |
| Popis: | Peripheral sensory neurons detect diverse physical stimuli and transmit the information into the CNS. At present, the genetic tools for specifically studying the development, plasticity, and regeneration of the sensory axon projections are limited. We found that the gene encoding Advillin, an actin binding protein that belongs to the gelsolin superfamily, is expressed almost exclusively in peripheral sensory neurons. We next generated a line of knock-in mice in which the start codon of theAdvillinis replaced by the gene encoding human placenta alkaline phosphatase (Avil-hPLAP mice). In heterozygous Avil-hPLAP mice, sensory axons, the exquisite sensory endings, as well as the fine central axonal collaterals can be clearly visualized with a simple alkaline phosphatase staining. Using this mouse line, we found that the development of peripheral target innervation and sensory ending formation is an ordered process with specific timing depending on sensory modalities. This is also true for the in-growth of central axonal collaterals into the brainstem and the spinal cord. Our results demonstrate that Avil-hPLAP mouse is a valuable tool for specifically studying peripheral sensory neurons. Functionally, we found that the regenerative axon growth ofAdvillin-null sensory neurons is significantly shortened and that deletion ofAdvillinreduces the plasticity of whisker-related barrelettes patterns in the hindbrain. |
| Databáze: | OpenAIRE |
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