The endometrium responds differently to cloned versus fertilized embryos
| Autor: | Thomas E. Spencer, Helmut Blum, Horst-Dieter Reichenbach, Megan Minten, M. Reichenbach, Stefan Bauersachs, Susanne E. Ulbrich, Valeri Zakhartchenko, Eckhard Wolf |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Nuclear Transfer Techniques Cloning Organism Placenta Embryonic Development Fertilization in Vitro Biology Endometrium Cell Line Andrology Pregnancy Internal medicine medicine Conceptus Animals Blastocyst RNA Messenger reproductive and urinary physiology Multidisciplinary urogenital system Gene Expression Profiling Placentation Gene Expression Regulation Developmental Embryo Biological Sciences Fibroblasts Embryo Mammalian Pregnancy Complications Endocrinology medicine.anatomical_structure embryonic structures Somatic cell nuclear transfer Oviduct Cattle Female |
| Popis: | Although somatic cell nuclear transfer (SCNT) cloning is more efficient in cattle than in any other species tested so far, there is a high rate of pregnancy failure that has been linked to structural and functional abnormalities of the placenta. We tested the hypothesis that these changes may originate from disturbed embryo–maternal interactions in the peri-implantation period. Therefore, we evaluated the response of the endometrium to SCNT embryos (produced from 7 different fetal fibroblast cell lines) as compared with embryos derived from in vitro fertilization (IVF). SCNT embryos and IVF embryos were cultured under identical conditions to the blastocyst stage (day 7) and were transferred to corresponding recipients, which were slaughtered at day 18 of pregnancy. The mRNA profiles of endometrium samples were obtained using a custom cDNA microarray enriched for transcripts differentially expressed in the endometrium and/or oviduct epithelium during the estrous cycle and/or early pregnancy. Overall, the variation in mRNA profiles was greater in the SCNT group than in the IVF group. Furthermore, 58 transcripts were differentially abundant in endometria from SCNT and IVF pregnancies. Prominent examples are orphan nuclear receptor COUP-TFII and connexin 43, both known to play important roles in uterine receptivity and conceptus placentation. These findings suggest that placental failure in bovine clone pregnancies may originate from abnormal embryo–maternal communication that develops during the peri-implantation period. Endometrium transcriptome profiles may serve as a tool to evaluate SCNT embryos for their ability to establish pregnancy and develop a functional placenta. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|