Autophagy is a major mechanism for the dual effects of curcumin on renal cell carcinoma cells
| Autor: | Quanhai Liu, Qian Deng, Yazhuo Jiang, Wanli Duan, Liang Liang, Liandong Zhang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Programmed cell death Curcumin Cell Survival Cell Antineoplastic Agents AMP-Activated Protein Kinases urologic and male genital diseases medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor medicine Autophagy Humans MTT assay Carcinoma Renal Cell Pharmacology Chemistry Oxidative Stress Pathway AMPK Endoplasmic Reticulum Stress Phenylbutyrates female genital diseases and pregnancy complications Kidney Neoplasms Acetylcysteine Oxidative Stress 030104 developmental biology medicine.anatomical_structure Pyrimidines 030220 oncology & carcinogenesis Cancer research Pyrazoles Oxidative stress Signal Transduction |
| Zdroj: | European journal of pharmacology. 826 |
| ISSN: | 1879-0712 |
| Popis: | The aim of this study was to explore the effects of curcumin on renal cell carcinoma（RCC） through regulating autophagy. Cell viabilities were determined by MTT assay in RCC cells after treatment with curcumin at different concentrations for various durations. ATG7 silencing RCC cells were established to test the role of autophagy. The levels of key proteins on autophagy pathway were analyzed by Western blot. We found out that following 24 h curcumin treatment, the viability of RCC cells had an increase at 5 μM and no significant change at 20 μM but a decrease at 80 μM. These effects were affected by the inhibition of autophagy. When pre-incubated with inhibitors of the AMPK and ER stress pathways, the LC3II levels of RCC cells at 5 μM and 20 μM of curcumin were significantly decreased; however, when treated with the inhibitor of the oxidative stress pathway, the LC3II levels of RCC cells at 80 μM were significantly decreased. In conclusion, the present study indicated Curcumin protected cells from death at low concentration but promotes cell death at high concentration. Autophagy played a dual role in curcumin's effects on RCC. The AMPK and ER stress pathways might be involved at low concentrations of curcumin to protect cells, while the oxidative stress pathway might take part in toxicity at high curcumin concentration. |
| Databáze: | OpenAIRE |
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