Microspectrofluorometry and fluorescence imaging in the study of human cytopathology
| Autor: | Cahide Kohen, Marco Monti, Vered Agmon, Joseph G. Hirschberg, Astrid Schachtschabel, Elli Kohen, Shimon Gatt, Dietrich O. Schachtschabel |
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| Rok vydání: | 2000 |
| Předmět: |
Senescence
Fluorescence-lifetime imaging microscopy Cellular pathology Histology Cellular differentiation Malates Biology Gluconates Fluorescence Mice Organelle Tumor Cells Cultured Animals Humans Dicarboxylic Acids Instrumentation Melanoma Cell Line Transformed Fluorescent Dyes Organelles beta-Glucosidase Cell Differentiation Fibroblasts NAD Cell biology Medical Laboratory Technology Spectrometry Fluorescence Cytopathology NAD+ kinase Lipid Peroxidation Anatomy Gene Deletion |
| Zdroj: | Microscopy research and technique. 51(5) |
| ISSN: | 1059-910X |
| Popis: | The study of energy pools and dynamics of specific pathways in living cells by microspectrofluorometry and fluorescence imaging produces spectral and topographic images characterizing structural and functional changes associated with cytopathology. Microspectro-fluorometry and fluorescence imaging have been applied, together with organelle morphometry to a number of cells mimicking certain cytopathologies, including melanoma cells, long-term malignant cells, and gene-defective cells. These investigations of cellular pathology indicate that there is a convergence of various physiopathological processes. Cellular states that have similarities include senescence, detoxification, and transformation. While the NAD(P)H metabolic transients have been studied before, our emphasis in this article is on very rapidly scanned fluorescence images related to organelle integration and photoinduced cellular senescence. Microsc. Res. Tech. 51:469–480, 2000. © 2000 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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