Generation of human embryonic stem cell line expressing a red fluorescent protein: BJNhem20-pCAG-tdTomato
| Autor: | Maneesha S. Inamdar, Deeti K. Shetty |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cellular differentiation Transgene Cell Genetic Vectors Human Embryonic Stem Cells Karyotype Embryoid body Biology Real-Time Polymerase Chain Reaction Transfection Green fluorescent protein Cell Line 03 medical and health sciences medicine Humans lcsh:QH301-705.5 Embryoid Bodies Medicine(all) Reverse Transcriptase Polymerase Chain Reaction Cell Differentiation General Medicine Cell Biology Molecular biology Embryonic stem cell Luminescent Proteins 030104 developmental biology medicine.anatomical_structure Microscopy Fluorescence lcsh:Biology (General) Cell culture Human embryonic stem cell line Transcription Factors Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 16, Iss 2, Pp 290-292 (2016) |
| ISSN: | 1876-7753 1873-5061 |
| Popis: | Human embryonic stem cell line BJNhem20-pCAG-tdTomato was generated using non-viral method. The construct pCAG-tdTomato was transfected using microporation procedure. This fluorescent hESC line can help to study heterogeneity within individual cells in hESC colonies by enabling live tracking of their growth, migration and differentiation properties. This cell line also serves as a resource for additional transgene introduction/knock-out/knock-in generation in a fluorescent background and allows ease of analysis in studies involving cell mixing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|