Composition and Protein Precipitation Capacity of Condensed Tannins in Purple Prairie Clover (Dalea purpurea Vent.)
| Autor: | L. Jin, Surya Acharya, Z. Xu, Yuxi Wang, Tianming Hu, Qianqian Huang, Irene Mueller-Harvey, Wayne E. Zeller, Tim A. McAllister |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
purple prairie clover Chemical structure Plant culture food and beverages Plant Science botanical distribution Dalea purpurea Vent SB1-1110 chemistry.chemical_compound Horticulture Benzyl mercaptan Proanthocyanidin chemistry Thiolysis chemical structure Protein precipitation Composition (visual arts) protein-precipitation capacity Condensed tannin condensed tannin Original Research |
| Zdroj: | Frontiers in Plant Science Frontiers in Plant Science, Vol 12 (2021) |
| ISSN: | 1664-462X |
| Popis: | This study aimed to determine the concentration and composition of condensed tannins (CT) in different tissues of purple prairie clover (PPC; Dalea purpurea Vent.) at different maturities and to determine their protein-precipitating capacity. The compositions of CT were elucidated after thiolysis with benzyl mercaptan followed by high-performance liquid-chromatography (HPLC) and 1H–13C heteronuclear single quantum coherence (HSQC) NMR spectroscopy. The results indicated that PPC flowering heads contained the highest CT concentration. Purple prairie clover CT consisted mainly of epicatechin (EC) and epigallocatechin (EGC) subunits. CT in the leaves were composed of more EC and less EGC than CT in stems and flowering heads at both the early flowering (EF) and late flowering (LF) head stages. The mean degree of polymerization was the highest for CT in stems and increased with maturity. CT isolated from PPC leaves at the early flowering head stage exhibited the greatest biological activity in terms of protein precipitation. Overall, the CT in PPC were predominantly procyanidins and the concentration and composition varied among the plant tissues and with maturity. |
| Databáze: | OpenAIRE |
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