Extracellular vesicle-coupled miRNA profiles in follicular fluid of cows with divergent post-calving metabolic status
Autor: | Christiane Neuhoff, Michael Hoelker, Samuel Gebremedhn, Mohammed Saeed-Zidane, Dawit Tesfaye, Tsige Hailay, Dessie Salilew-Wondim, Mikhael Poirier, Christina M Dauben, Karl Schellander, Ernst Tholen, Franca Rings |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Ice calving lcsh:Medicine Biology Article Andrology 03 medical and health sciences Extracellular Vesicles 0302 clinical medicine Lactation microRNA Follicular phase medicine Animals lcsh:Science 2. Zero hunger Multidisciplinary Molecular medicine Gene Expression Profiling Postpartum Period lcsh:R Functional genomics Extracellular vesicle Oocyte Follicular fluid Follicular Fluid Metabolic pathway MicroRNAs 030104 developmental biology medicine.anatomical_structure Metabolome Cattle Female lcsh:Q Energy Metabolism 030217 neurology & neurosurgery |
Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Most high-yielding dairy cows enter a state of negative energy balance (NEB) during early lactation. This, in turn, results in changes in the level of various metabolites in the blood and follicular fluid microenvironment which contributes to disturbed fertility. Extracellular vesicles (EVs) are evolutionarily conserved communicasomes that transport cargo of miRNA, proteins and lipids. EV-coupled miRNAs have been reported in follicular fluid. However, the association between postpartum NEB and EV-coupled miRNA signatures in follicular fluid is not yet known. Energy balance analysis in lactating cows shortly after post-calving revealed that the majority of the cows exhibited transiently negative energy balance levels, whereas the remaining cows exhibited either consistently negative or consistently positive energy levels. Metabolic status was associated with EV-coupled miRNA composition in the follicular fluid. Cows experiencing NEB showed reduced expression of a large number of miRNAs while cows with positive energy balances primarily exhibited elevated expression of EV-coupled miRNAs. The miRNAs that were suppressed under NEB were found to be involved in various metabolic pathways. This is the first study to reveal the presence of an association between EV-coupled miRNA in follicular fluid and metabolic stress in dairy cows. The involvement of differentially expressed miRNAs in various pathways associated with follicular growth and oocyte maturation suggest the potential involvement of specific follicular miRNAs in oocyte developmental competence, which may partially explain reduced fertility in cows due to post-calving metabolic stress. |
Databáze: | OpenAIRE |
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