Detection of Minimal Residual Disease in B Lymphoblastic Leukemia by High-Throughput Sequencing of IGH
| Autor: | Ilan R. Kirsch, Christopher S. Carlson, Mark J. Rieder, Bryan Howie, Harlan Robins, Anne L. Angiolillo, Anna Sherwood, Jennifer Vogt, Brent L. Wood, David Williamson, David Wu, Ryan O. Emerson, Mignon L. Loh |
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| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
Neoplasm Residual Gene Rearrangement B-Lymphocyte Heavy Chain Somatic evolution in cancer Article Disease-Free Survival DNA sequencing Flow cytometry hemic and lymphatic diseases Leukemia B-Cell medicine Humans medicine.diagnostic_test biology High-Throughput Nucleotide Sequencing Cancer Gene rearrangement Flow Cytometry Prognosis medicine.disease Molecular biology Minimal residual disease body regions Leukemia Oncology Immunology biology.protein Antibody Immunoglobulin Heavy Chains |
| Zdroj: | Clinical Cancer Research. 20:4540-4548 |
| ISSN: | 1557-3265 1078-0432 |
| DOI: | 10.1158/1078-0432.ccr-13-3231 |
| Popis: | Purpose: High-throughput sequencing (HTS) of immunoglobulin heavy-chain genes (IGH) in unselected clinical samples for minimal residual disease (MRD) in B lymphoblastic leukemia (B-ALL) has not been tested. As current MRD-detecting methods such as flow cytometry or patient-specific qPCR are complex or difficult to standardize in the clinical laboratory, sequencing may enhance clinical prognostication. Experimental Design: We sequenced IGH in paired pretreatment and day 29 post-treatment samples using residual material from consecutive, unselected samples from the Children's Oncology Group AALL0932 trial to measure MRD as compared with flow cytometry. We assessed the impact of ongoing recombination at IGH on MRD detection in post-treatment samples. Finally, we evaluated a subset of cases with discordant MRD results between flow cytometry and sequencing. Results: We found clonal IGH rearrangements in 92 of 98 pretreatment patient samples. Furthermore, while ongoing recombination of IGH was evident, index clones typically prevailed in MRD-positive post-treatment samples, suggesting that clonal evolution at IGH does not contribute substantively to tumor fitness. MRD was detected by sequencing in all flow cytometry–positive cases with no false-negative results. In addition, in a subset of patients, MRD was detected by sequencing, but not by flow cytometry, including a fraction with MRD levels within the sensitivity of flow cytometry. We provide data that suggest that this discordance in some patients may be due to the phenotypic maturation of the transformed cell. Conclusion: Our results provide strong support for HTS of IGH to enhance clinical prognostication in B-ALL. Clin Cancer Res; 20(17); 4540–8. ©2014 AACR. |
| Databáze: | OpenAIRE |
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