Using enzyme cascades in biocatalysis: Highlight on transaminases and carboxylic acid reductases
| Autor: | Michail N. Isupov, Jennifer A. Littlechild, Nicholas J. Harmer, Rhys Cutlan, Simone Antonio De Rose |
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| Rok vydání: | 2019 |
| Předmět: |
Green chemistry
Carboxylic acid Microfluidics Biophysics Coenzymes 010402 general chemistry 01 natural sciences Biochemistry Cofactor Analytical Chemistry Molecular Biology Transaminases chemistry.chemical_classification biology Carboxylic acid reductase 010405 organic chemistry Industrial scale Green Chemistry Technology Combinatorial chemistry 0104 chemical sciences Kinetics Enzyme chemistry Biocatalysis biology.protein Oxidoreductases Flux (metabolism) |
| Zdroj: | Biochimica et biophysica acta. Proteins and proteomics. 1868(2) |
| ISSN: | 1878-1454 |
| Popis: | Biocatalysis, the use of enzymes in chemical transformations, is an important green chemistry tool. Cascade reactions combine different enzyme activities in a sequential set of reactions. Cascades can occur within a living (usually bacterial) cell; in vitro in 'one pot' systems where the desired enzymes are mixed together to carry out the multi-enzyme reaction; or using microfluidic systems. Microfluidics offers particular advantages when the product of the reaction inhibits the enzyme(s). In vitro systems allow variation of different enzyme concentrations to optimise the metabolic 'flux', and the addition of enzyme cofactors as required. Cascades including cofactor recycling systems and modelling approaches are being developed to optimise cascades for wider industrial scale use. Two industrially important enzymes, transaminases and carboxylic acid reductases are used as examples regarding their applications in cascade reactions with other enzyme classes to obtain important synthons of pharmaceutical interest. |
| Databáze: | OpenAIRE |
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