Two-photon excited fluorescence of silica nanoparticles loaded with a fluorene-based monomer and its cross-conjugated polymer: their application to cell imaging
| Autor: | Víctor M. Tellez-Lopez, Mikhail G. Zolotukhin, Serguei Fomine, Oracio Barbosa-García, Mario Rodríguez, J. L. Pichardo-Molina, Daniel Martinez-Fong, Laura Aparicio-Ixta, M. A. Meneses-Nava, José-Luis Maldonado, Gabriel Ramos-Ortiz |
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| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_classification
Fluorenes Photons Materials science Cell Survival Polymers Silicon dioxide Nanoparticle Quantum yield Polymer Fluorene Silicon Dioxide Photochemistry Fluorescence chemistry.chemical_compound Monomer Microscopy Fluorescence chemistry Cell Line Tumor Fluorescence microscope Humans Nanoparticles Organic chemistry General Materials Science HeLa Cells |
| Zdroj: | Nanoscale. 4:7751 |
| ISSN: | 2040-3372 2040-3364 |
| DOI: | 10.1039/c2nr31925j |
| Popis: | In this work the two-photon activity of nanoparticles obtained from a fluorene monomer (M1) and its cross-conjugated polymer (P1) is reported. Aqueous suspensions of M1 and P1 nanoparticles prepared through the reprecipitation method exhibited maximum two-photon absorption (TPA) cross-sections of 84 and 9860 GM (1 GM = 10(-50) cm(4) s) at 740 nm, respectively, and a fluorescence quantum yield of ~1. Such a two-photon activity was practically equal with respect to that for molecular solutions of M1 and P1. These materials were then successfully encapsulated into silica nanoparticles to provide bio-compatibly. A lung cancer cell line (A549) and a human cervical cancer cell line (HeLa cells) were incubated with our fluorescent silica nanoparticles to carry out two-photon imaging. By means of these studies we demonstrate that optimized nonlinear optical polymers loaded in silica nanoparticles can be used as efficient probes with low cytotoxicity and good photostability for two-photon fluorescence microscopy. To the best of our knowledge, studies concerning polymer-doped silica nanoparticles exhibiting large two-photon activity have not been reported in the literature. |
| Databáze: | OpenAIRE |
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