Metabolism of methyl-branched iodo palmitic acids in cultured hepatocytes
| Autor: | Jean Chambaz, Sabine Coornaert, Dominique Pepin, Michel Vidal, Marcel Apparu, Ginette Thomas, Gilbert Bereziat, Claude Loriette |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Male
animal structures Primary culture Palmitic Acids In Vitro Techniques Iodine Radioisotopes Palmitic acid chemistry.chemical_compound In vivo medicine Animals heterocyclic compounds Radiology Nuclear Medicine and imaging Secretion Incubation Cells Cultured chemistry.chemical_classification Chemistry virus diseases Fatty acid General Medicine Metabolism Rats medicine.anatomical_structure Liver Biochemistry Hepatocyte |
| Zdroj: | European Journal of Nuclear Medicine. 15:367-372 |
| ISSN: | 1619-7089 0340-6997 |
| DOI: | 10.1007/bf00449226 |
| Popis: | The metabolic fate of methyl-branched iodo fatty acids was studied in primary culture of rat hepatocytes. We compared 16-iodo-2-R,S-methyl palmitic acid (2-Me), which can be β oxidized, with 16-iodo-3-R,S-methyl palmitic acid (3-Me) which can be β oxidized only after an initial α oxydation and with 16-iodo-2,2-dimethyl palmitic acid (2,2-Me2) and 16-iodo-3,3-dimethyl palmitic acid (3,3-Me2) which cannot be β oxidized at all. The normal fate of natural fatty acids was given by comparative experiments with [1-14C] palmitic acid. Monomethyl-branched iodo fatty acids were taken up in the same range as palmitic acid but more than dimethyl-branched iodo fatty acids. After a 15-h incubation, acido-soluble products (ASP) accounted for 75% of the radioactivity taken up as 16-iodo-2-methyl palmitic acid, 50% as other methyl-branched iodo fatty acids and only 30% as palmitic acid, which indicated that all the methyl-branched iodo fatty acids underwent a strong deiodination process. Fatty acids were esterified in the following order: palmitic acid >16-iodo-3-R,S-methyl palmitic acid>16-iodo-2-R,S-methyl palmitic acid>16-iodo-2,2-dimethyl palmitic acid>16-iodo-3,3-dimethyl palmitic acid. Cultured hepatocytes, labelled for 3 h with the various fatty acids and reincubated for 12 h without fatty acid, secreted large amounts of free dimethylbranched iodo fatty acids as compared to the monomethyl ones and palmitic acid. Only hepatocytes prelabelled with 16-[125I]iodo-2,2-dimethyl palmitic acid exhibited an appreciable secretion of labeled triglycerides, but at a lower rate than with [1-14C] palmitic acid. Conversely, the 16-iodo-monomethyl palmitic acids remained chiefly in hepatocyte triglycerides. Minute amounts of 16-iodo-methyl-branched-palmitic acids were found in hepatocyte or secred phospholipids as compared with palmitic acid. This metabolic fate of methyl-branched iodo palmitic acids argues against their utilization as imaging probes to monitor in vivo the synthesis and the secretion of triglycerides by the liver. |
| Databáze: | OpenAIRE |
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