Molecular Epidemiology of Hepatitis C Virus (HCV) in Kadun State
| Autor: | Z Sheyin, AI Mamman, AJ Randawa, BD Jatau |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Molecular epidemiology
biology business.industry General Chemical Engineering Hepatitis C virus virus diseases medicine.disease_cause Virology digestive system diseases Reverse transcriptase law.invention law Genotype Immunology biology.protein Medicine Outpatient clinic Antibody business Genotyping Polymerase chain reaction |
| Zdroj: | African Journal of Clinical and Experimental Microbiology; Vol 13, No 2 (2012); 61-65 |
| ISSN: | 1595-689X |
| Popis: | Objective: To determine the distribution of hepatitis C virus (HCV) genotypes and subtypes among blood donors and outpatients attendees positive for antibody to HCV (anti-HCV). Justification: Hepatitis C virus (HCV) continues to be a major disease burden on the world and Man is the only known natural host of Hepatitis C virus (Chivaliez and Pawlotsky, 2007). There is no published data on the prevalence of the genotypes and subtypes of HCV in Kaduna State. Setting: Three hospitals one in each of the 3 senatorial zones in Kaduna State. Patients: Blood donors who reported for blood donation and outpatient department attendees. Method: Antibody detection by a third generation HCV ELISA (Biotech Laboratories, UK); HCV RNA and genotyping by Reverse Transcriptase polymerase chain reaction with genotype-specific primers. (Sacace Biotechnologies, UK). Results: of the 259 plasma specimens screened for Hepatitis C virus in this study, 20(7.7%) were positive for anti-HCV antibodies by ELISA and 16(6.2%) of the antibodies positive specimen were positive for HCV RNA. Of the 139 blood donors tested, 8 (5.8%) were HCV RNA positive. Similarly, 120 were tested from the outpatient Department attendees and 8 (6.7%) were HCV RNA positive. Hepatitis C virus genotype 1b was found in the entire HCV RNA positive sample. Conclusions: The findings of 6.2% prevalence of HCV infection based on HCV RNA test confirmed that there is Hepatitis C virus in Kaduna State with genotype 1b as the predominant genotype found in all the three senatorial zones. |
| Databáze: | OpenAIRE |
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