Preparative capillary electrophoresis based on adsorption of the solutes (proteins) onto a moving blotting membrane as they migrate out of the capillary

Autor:	Stellan Hjertén, Anders Palm, Kjell-Ove Eriksson
Rok vydání:	1992
Předmět:	Electrophoresis Chromatography Chemistry Capillary action Polyvinylidene difluoride Biophysics Collodion Proteins Membranes Artificial Cell Biology Mass spectrometry Biochemistry Blot Membrane Adsorption Capillary electrophoresis Animals Humans Molecular Biology
Zdroj:	Analytical biochemistry. 201(2)
ISSN:	0003-2697
Popis:	A micropreparative capillary electrophoresis apparatus equipped with a new type of fraction collection device is described: solutes, such as proteins, are adsorbed onto a moving blotting membrane (for instance a polyvinylidene difluoride membrane) as they migrate electrophoretically out of the capillary. The adsorbed proteins are visualized by conventional protein staining methods or be fluorescent labeling. Specific identification of separated components by an immunological technique is demonstrated. The method also offers the potential to analyze proteins and peptides collected on the membrane by gas phase sequencing and mass spectrometry.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b129eac27146b0172e1779d95eef5a7a https://pubmed.ncbi.nlm.nih.gov/1632508 Zobrazit plný text záznamu