Modulation of response to radiation of human lung cancer cells following insulin-like growth factor 1 receptor inactivation

Autor: Rolf Lewensohn, Jessica Ekedahl, Lena Kanter, Anica Dricu, Juan Castro, Mia Carapancea, Daria Cosaceanu
Rok vydání: 2005
Předmět:
Zdroj: Cancer Letters. 222:173-181
ISSN: 0304-3835
DOI: 10.1016/j.canlet.2004.10.002
Popis: Targeted disruption of the insulin-like growth factor 1 receptor (IGF-1R) restricts proliferation of tumor cells and enhances their in vitro radiosensitivity. However, there is little information regarding the effect of IGF-1R expression and function on the lung cancer response to radiotherapy. In this study, we evaluated the cell surface expression of IGF-1R and the antitumoral effect of IGF-1R blockade in combination with irradiation in 6 non-small cell lung cancer (NSCLC) cell lines. All cell lines showed specific IGF-1 binding with an affinity ranging from 0.95×10 −9 to 2.3×10 −9 M, which was evaluated by competitive binding assay. The amount of binding sites ranged from 118 to 377 fmol/mg protein. In one cell line (U1810), the combined treatment led to synergistic cell death and was associated with an accumulation of cells in the G 2 phase. IGF-1R activation was able to obstruct serum starvation/radiation-induced cell death in U1810 cell line. Additive interactions were found for four cell lines (A549, H157, H23 and H125) whereas only subadditive effects were observed in U1752 cell line. Our results indicate that the IGF-1R is present on NSCLC cells and thereby its involvement in the modulation of radiosensitivity in lung cancer cells.
Databáze: OpenAIRE
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