pPAC-ResQ: A Yeast–Bacterial Shuttle Vector for Capturing Inserts from P1 and PAC Clones by Recombinogenic Targeted Cloning
| Autor: | Chris T. Amemiya, Frank H. Ruddle, Kevin L. Bentley, Cooduvalli S. Shashikant, Jaya Bhargava, Janet L. Carr |
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| Rok vydání: | 1999 |
| Předmět: |
Genetics
Yeast artificial chromosome Cloning Bacteria biology Genetic Vectors Molecular Sequence Data Saccharomyces cerevisiae Genetic transfer Computational biology Molecular cloning biology.organism_classification Models Biological chemistry.chemical_compound chemistry Shuttle vector Yeasts Cloning Molecular Homologous recombination DNA Gene Library |
| Zdroj: | Genomics. 56:337-339 |
| ISSN: | 0888-7543 |
| DOI: | 10.1006/geno.1998.5710 |
| Popis: | We have developed a method to capture inserts from P1 and P1 artificial chromosome (PAC) clones into a yeast–bacteria shuttle vector by using recombinogenic targeting. We have engineered a vector, pPAC-ResQ, a derivative of pClasper, which was previously used to capture inserts from yeast artificial chromosome clones. pPAC-ResQ contains DNA fragments flanking the inserts in P1 and PAC vectors as recombinogenic ends. When linearized pPAC-ResQ vector and P1 or PAC DNA are cotransformed into yeast, recombination between the two leads to the transfer of inserts into pPAC-ResQ. pPAC-ResQ clones thus obtained can be further modified in yeast for functional analysis and shuttled to Escherichia coli to produce large quantities of cloned DNA. This approach provides a rapid method to modify P1/PAC clones for functional analysis. |
| Databáze: | OpenAIRE |
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