Long-Term Recovery After Endothelial Colony-Forming Cells or Human Umbilical Cord Blood Cells Administration in a Rat Model of Neonatal Hypoxic-Ischemic Encephalopathy

Autor: Alaa Ramdani, Françoise Dignat-George, Florence Sabatier, Benjamin Guillet, Isabelle Grandvuillemin, Farid Boubred, Umberto Simeoni, Philippe Garrigue
Přispěvatelé: Division of Neonatology, La Conception Hospital, Assistance Publique-Hôpitaux de Marseille and Faculté de Médecine, Université de la Méditerranée - Aix-Marseille 2, Vascular research center of Marseille (VRCM), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Nutrition, obésité et risque thrombotique (NORT), Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital de la Conception [CHU - APHM] (LA CONCEPTION ), Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital de la Conception [CHU - APHM] (LA CONCEPTION)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Endothelial colony‐forming cells
Pathology
Vascular Regeneration
Cord Blood
[SDV]Life Sciences [q-bio]
Perfusion scanning
Umbilical cord
Umbilical Cord
Rats
Sprague-Dawley
Cell therapy
0302 clinical medicine
Translational Research Articles and Reviews
Medicine
Neonatal hypoxic ischemic encephalopathy
Cells
Cultured
Cord / Cord Blood Stem Cells (WJ‐MSCs)
Endothelial Progenitor Cells
Animals
Brain/blood supply
Brain/diagnostic imaging
Brain/physiopathology
Cerebrovascular Circulation
Endothelial Progenitor Cells/cytology
Humans
Hypoxia-Ischemia
Brain/therapy
Mesenchymal Stem Cell Transplantation/methods
Rats
Umbilical Cord/cytology
Endothelial colony-forming cells
Human umbilical cord blood cells
Rat model
Single photon emission computed tomography
Brain
General Medicine
3. Good health
medicine.anatomical_structure
Cerebral blood flow
Anesthesia
Hypoxia-Ischemia
Brain
Stem cell
medicine.medical_specialty
Encephalopathy
Mesenchymal Stem Cell Transplantation
03 medical and health sciences
Progenitor cell
business.industry
Cell Biology
medicine.disease
Transplantation
030104 developmental biology
business
Ischemia / Reperfusion Injury
030217 neurology & neurosurgery
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Developmental Biology
Zdroj: Stem Cells Translational Medicine
Stem Cells Translational Medicine, Wiley, 2017, 6 (11), pp.1987-1996. ⟨10.1002/sctm.17-0074⟩
Stem cells translational medicine, vol. 6, no. 11, pp. 1987-1996
ISSN: 2157-6564
2157-6580
DOI: 10.1002/sctm.17-0074⟩
Popis: International audience; Neonatal hypoxic-ischemic encephalopathy (NHIE) is a dramatic perinatal complication, associated with poor neurological prognosis despite neuroprotection by therapeutic hypothermia, in the absence of an available curative therapy. We evaluated and compared ready-to-use human umbilical cord blood cells (HUCBC) and bankable but allogeneic endothelial progenitors (ECFC) as cell therapy candidate for NHIE. We compared benefits of HUCBC and ECFC transplantation 48 hours after injury in male rat NHIE model, based on the Rice-Vannucci approach. Based on behavioral tests, immune-histological assessment and metabolic imaging of brain perfusion using single pho-ton emission computed tomography (SPECT), HUCBC, or ECFC administration provided equally early and sustained functional benefits, up to 8 weeks after injury. These results were associated with total normalization of injured hemisphere cerebral blood flow assessed by SPECT/CT imaging. In conclusion, even if ECFC represent an efficient candidate, HUCBC autologous criteria and easier availability make them the ideal candidate for hypoxic-ischemic cell therapy. STEM CELLS TRANSLATIONAL Neonatal hypoxic ischemic encephalopathy is a dramatic perinatal complication. Neurological and neurosensory sequelae are frequent in survivors, including motor or learning disabilities, cerebral palsy, or epilepsy. Facing the absence of effective curative therapy, many hopes have been credited in cell therapy strategies. Based on behavioral tests, immune-histological assessment and metabolic imaging of brain perfusion using single photon emission computed tomog-raphy, we report in this work that cell transplantation of both human umbilical cord blood cells and endothelial progenitors provided equally early and sustained functional benefits, up to adulthood.
Databáze: OpenAIRE
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