Enzymatic Interconversion of Isomorphic Fluorescent Nucleosides: Adenosine Deaminase Transforms an Adenosine Analogue into an Inosine Analogue
| Autor: | Renatus W. Sinkeldam, Lisa S. McCoy, Dongwon Shin, Yitzhak Tor |
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| Rok vydání: | 2013 |
| Předmět: |
Adenosine
Adenosine Deaminase Stereochemistry Purine nucleoside phosphorylase high-throughput screening Catalysis Adenosine deaminase medicine Purine metabolism Inosine biology Chemistry Organic Chemistry Nucleosides AMP deaminase General Medicine General Chemistry adenosine deamination Biochemistry kinetics Chemical Sciences biology.protein fluorescence Adenosine Deaminase Inhibitor Nucleoside medicine.drug |
| Zdroj: | Angewandte Chemie (International ed. in English), vol 52, iss 52 |
| ISSN: | 1433-7851 |
| DOI: | 10.1002/anie.201307064 |
| Popis: | Adenosine deaminase, a major enzyme involved in purine metabolism, converts an isomorphic fluorescent analogue of adenosine (thA) to an isomorphic inosine analogue (thI), which possesses distinct spectral features, allowing one to monitor the enzyme-catalyzed reaction and its inhibition in real time. The utility of this sensitive fluorescently-monitored transformation for the high throughput detection and analysis of ADA inhibitors is demonstrated. |
| Databáze: | OpenAIRE |
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